. "Citraconylation - a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry" . . . "P(GA203/02/0922)" . . "BE - Belgick\u00E9 kr\u00E1lovstv\u00ED" . . "Kod\u00ED\u010Dek, Milan" . . "601327" . "Citraconylation - a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry"@en . . "Lysine ?-amino group reacts with citraconic anhydride forming a derivative which is stable on terms for trypsin cleavage. This modification changes the spectrum of peptides formed by the trypsin action; as the number of trypsin-sensitive sites is reduced, the peptides with higher molecular mass can survive in the digest. The various studies of proteins by MALDI-TOF mass spectrometry are often complicated by the low sequence coverage of the peptide chain. This paper demonstrates that the modification of proteins by citraconylation before trypsin cleavage represents a simple experimental technique which allows a significant increase of sequence coverage in MALDI-TOF mass spectrometry. This improvement is caused both by change of trypsin fragmentation pattern and by disturbance of the protein's native tertiary structure."@en . "3"^^ . "RIV/60461373:22330/03:00008939!RIV/2004/GA0/223304/N" . "61" . "22330" . "RIV/60461373:22330/03:00008939" . . . "0006-291X" . "305" . "1091-1093" . . . "[657530158DF5]" . "3"^^ . "0"^^ . "Kadl\u010D\u00EDk, Vojt\u011Bch" . "0"^^ . "3"^^ . . "Biochemical and Biophysical Research Communications" . "Strohalm, Martin" . . "Citraconylation - a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry"@en . . . . "Lysine ?-amino group reacts with citraconic anhydride forming a derivative which is stable on terms for trypsin cleavage. This modification changes the spectrum of peptides formed by the trypsin action; as the number of trypsin-sensitive sites is reduced, the peptides with higher molecular mass can survive in the digest. The various studies of proteins by MALDI-TOF mass spectrometry are often complicated by the low sequence coverage of the peptide chain. This paper demonstrates that the modification of proteins by citraconylation before trypsin cleavage represents a simple experimental technique which allows a significant increase of sequence coverage in MALDI-TOF mass spectrometry. This improvement is caused both by change of trypsin fragmentation pattern and by disturbance of the protein's native tertiary structure." . "Citraconylation - a simple method for high protein sequence coverage in MALDI-TOF mass spectrometry" . . "MALDI-TOF mass spectrometry, protein sequence coverage, citraconylation"@en .