"81-82" . "Czech Journal of Food Science" . "RIV/60461373:22330/00:00013228" . . . "2"^^ . . "Skulinov\u00E1, Michaela" . "Changes of trypsin inhibitor activity during microwave treatment of germinated pea" . "22330" . "Ke stanoven\u00ED aktivity trypsin inhibitoru (TIA) byla pou\u017Eita metoda se syntetick\u00FDm substr\u00E1tem BAPNA (N--benzoyl-DL-arginin-p-nitroanilid). K extrakci b\u00EDlkovin byl pou\u017Eit glycinov\u00FD pufr pH 11 obsahuj\u00EDc\u00ED mo\u010Dovinu a EDTA, 2 h m\u00EDch\u00E1n\u00ED a odst\u0159e\u010Fov\u00E1n\u00ED. Syntetick\u00FD substr\u00E1t BAPNA je hydrolyzov\u00E1n trypsinem a m\u011B\u0159\u00ED se absorbance \u017Elut\u00E9ho p-nitroanilidu p\u0159i 410 nm. Byly vyhodnoceny zm\u011Bny TIA b\u011Bhem kl\u00ED\u010Den\u00ED a n\u00E1sleduj\u00EDc\u00EDho mikrovlnn\u00E9ho oh\u0159evu a p\u0159i konven\u010Dn\u00EDm su\u0161en\u00ED nakl\u00ED\u010Den\u00E9ho hrachu do su\u0161iny 12 - 14 %."@cs . . . "Zm\u011Bna aktivityTrypsin-inhibitoru b\u011Bhem mikrovlnn\u00E9ho oh\u0159evu nakl\u00ED\u010Den\u00E9ho hrachu"@cs . . . . . "[BB9BC9A47809]" . "18" . . . "CZ - \u010Cesk\u00E1 republika" . "1212-1800" . . "2"^^ . "The method by means synthetic substrate BAPNA (N--benzoyl-DL-arginin-p-nitroanilid) was used for trypsin-inhibitor activity (TIA) determination in pea seeds. To the extraction of proteins is used glycine buffer pH 11 containing urea and EDTA, 2 h stirring and centrifugation. Synthetic substrate BAPNA is hydrolysed by trypsin and the absorbance of dislodged yellow p-nitroanilid is measured at 410 nm. The changes of TIA during germination and the following microwave treatment and the conventional drying of germinated pea to the final moisture 12 - 14 % were evaluated." . "P(OK 325), Z(MSM 223300005)" . "RIV/60461373:22330/00:00013228!RIV06-MSM-22330___" . "Kadlec, Pavel" . . "pea; germination; microwave treatment; trypsin-inhibitor activity"@en . "Changes of trypsin inhibitor activity during microwave treatment of germinated pea"@en . "Changes of trypsin inhibitor activity during microwave treatment of germinated pea" . "Zm\u011Bna aktivityTrypsin-inhibitoru b\u011Bhem mikrovlnn\u00E9ho oh\u0159evu nakl\u00ED\u010Den\u00E9ho hrachu"@cs . . . "706523" . "The method by means synthetic substrate BAPNA (N--benzoyl-DL-arginin-p-nitroanilid) was used for trypsin-inhibitor activity (TIA) determination in pea seeds. To the extraction of proteins is used glycine buffer pH 11 containing urea and EDTA, 2 h stirring and centrifugation. Synthetic substrate BAPNA is hydrolysed by trypsin and the absorbance of dislodged yellow p-nitroanilid is measured at 410 nm. The changes of TIA during germination and the following microwave treatment and the conventional drying of germinated pea to the final moisture 12 - 14 % were evaluated."@en . . "2"^^ . . . "Changes of trypsin inhibitor activity during microwave treatment of germinated pea"@en . .