. . "RIV/60460709:_____/04:01:_3621!RIV06-MZE-60460709" . "Polyclonal antibodies pro detekci moucn\u00E9ho cerva Acarus siro"@cs . "Polyclonal antibodies for detection of flour mite Acarus siro"@en . . "9"^^ . . "580173" . "Polyclonal antibodies pro detekci moucn\u00E9ho cerva Acarus siro"@cs . "Ry\u0161\u00E1nek, Pavel" . . "Stejskal, V\u00E1clav" . "Polyclonal antibodies for detection of flour mite Acarus siro" . . . . "Polyclonal antibodies for detection of flour mite Acarus siro" . "03" . . "Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need for" . "1"^^ . "RIV/60460709:_____/04:01:_3621" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "Polyclonal antibodies for detection of flour mite Acarus siro"@en . "Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need for"@en . . . . "Chalupn\u00EDkov\u00E1, Julie" . . "14" . "5"^^ . "Phytophaga 14" . "Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need forrapid detection methods such as ELISA. The aim of our work was therefore to prepare and characterise antibodies for detection of the flour mite (Acarus siro). Two polyclonal antibodies (anti- A.siroK53 and anti-A.siroK54) were prepared against an antigen in the form of protein fraction from whole body homogenates of Acarus siro. Specificity and sensitivity of the prepared antibodies were evaluated. We did not observe any cross-reactivity with stored beetles, moths, other mite species, micro-fungi, therearing diet of mites (yeast and wheat diet) and extracts from wheat kernels. Cross-reactivity to Acarus gracilis was found out. This phenomenon is discussed in relation to the evolution position of this species. The obtained antibodies (anti-A.siroK53 a"@cs . "ELISA, PCR, cereal products, food, monitoring, detection"@en . . "Kudl\u00EDkov\u00E1, Iva" . "[8A837ABAD046]" . "P(QF4071)" . "Hubert, Jan" . . . "711-720" .