"Rostlinn\u00E1 v\u00FDroba (Plant Production)" . "RIV/60460709:_____/02:00004302" . . . . . . . "4"^^ . "Sedl\u00E1k, Petr" . "Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism" . . "P(EP9107), Z(MSM 412100002)" . . "648291" . "48" . . "RIV/60460709:_____/02:00004302!RIV/2003/MZE/410003/N" . "Vejl, Pavel" . . . . . . "0370-663X" . "[F4DDBD9A06D0]" . "CZ - \u010Cesk\u00E1 republika" . . "Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism"@en . . . "Globodera rostochiensis, Globodera pallida, ITS - 1 region, PCR, DNA markery, identifikace druhu"@en . "Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism" . "Skupinov\u00E1, Sylva" . "3"^^ . "11" . "Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism"@en . "Actual methods of molecular analyses of DNA are routinely used at fields of zoological and botanical taxonomy, pest management and plant breeding. Knowledge of species-composition in populations of potato cyst nematodes (Globodera rostochiensis, G. pallida) is very important for selection of appropriate measure of regulation PCN's occurrence. The molecular method for distinguishing of PCN species is described at this article. There were used European populations of PCN - \u0160luknov (Ro1), Obersteinbach (Ro2), Harmerz (Ro5), Kalle (Pa2), Chavornay (Pa3), Delmsen (Pa3), and some cysts of unknown patotype from Ka\u0161persk\u00E9 hory (K) locality. Species-specific sets of primers for ITS-1 (Internal Transcribed Spacer 1) amplification were designed on base of known sequences ITS-1 of both PCN species by using of freeware Primers! for the World Wide Web. By using of set Fro1 - Rro1 was product 411bp detected (only in cause G. rostochiensis), by using of set Fpa2 - Rpa1 the product 239bp was detected (only G. pallida)" . "Domk\u00E1\u0159ov\u00E1, J." . . "0"^^ . "4"^^ . "Actual methods of molecular analyses of DNA are routinely used at fields of zoological and botanical taxonomy, pest management and plant breeding. Knowledge of species-composition in populations of potato cyst nematodes (Globodera rostochiensis, G. pallida) is very important for selection of appropriate measure of regulation PCN's occurrence. The molecular method for distinguishing of PCN species is described at this article. There were used European populations of PCN - \u0160luknov (Ro1), Obersteinbach (Ro2), Harmerz (Ro5), Kalle (Pa2), Chavornay (Pa3), Delmsen (Pa3), and some cysts of unknown patotype from Ka\u0161persk\u00E9 hory (K) locality. Species-specific sets of primers for ITS-1 (Internal Transcribed Spacer 1) amplification were designed on base of known sequences ITS-1 of both PCN species by using of freeware Primers! for the World Wide Web. By using of set Fro1 - Rro1 was product 411bp detected (only in cause G. rostochiensis), by using of set Fpa2 - Rpa1 the product 239bp was detected (only G. pallida)"@en . "486-489" . "0"^^ . . . . .