. . . "Koko\u0161ka, Ladislav" . "NL - Nizozemsko" . . "2"^^ . "The broth microdilution (BMD) method is widely used for the determination of minimum inhibitory concentrations of antimicrobial agents, including volatile oils and their components. In this series of various experiments, we have demonstrated the influence of thymoquinone (TQ) vapor on the results of the BMD test performed with Staphylococcus aureus as a model organism. The spread of vapor from the TQ containing wells (32\u2013512 \u00B5g/mL) caused the complete inhibition of staphylococcal growth in adjoining wells initially containing bacterium-inoculated pure Mueller\u2013Hinton broth only and thus produced false positive results of the test. The ability of TQ to pass into the adjoined wells was subsequently confirmed by gas chromatography\u2013mass spectrometry, whereas TQ at concentrations up to 84 \u00B5g/mL was detected in these wells after five hours. Based on these results, we suppose that vapors of TQ as well as of other naturally occurring volatile compounds and their mixtures (for example essential oils and plant"@en . "Havl\u00EDk, Jaroslav" . . . "Rondevaldov\u00E1, Johana" . "The broth microdilution (BMD) method is widely used for the determination of minimum inhibitory concentrations of antimicrobial agents, including volatile oils and their components. In this series of various experiments, we have demonstrated the influence of thymoquinone (TQ) vapor on the results of the BMD test performed with Staphylococcus aureus as a model organism. The spread of vapor from the TQ containing wells (32\u2013512 \u00B5g/mL) caused the complete inhibition of staphylococcal growth in adjoining wells initially containing bacterium-inoculated pure Mueller\u2013Hinton broth only and thus produced false positive results of the test. The ability of TQ to pass into the adjoined wells was subsequently confirmed by gas chromatography\u2013mass spectrometry, whereas TQ at concentrations up to 84 \u00B5g/mL was detected in these wells after five hours. Based on these results, we suppose that vapors of TQ as well as of other naturally occurring volatile compounds and their mixtures (for example essential oils and plant" . "0367-326X" . . "6"^^ . "50502" . "N" . "41340" . "Klou\u010Dek, Pavel" . . "6"^^ . . . . "Nov\u00FD, Pavel" . "Thymoquinone vapor significantly affects the results of Staphylococcus aureus sensitivity tests using the standard broth microdilution method"@en . . "94" . "000335608700013" . "Thymoquinone vapor significantly affects the results of Staphylococcus aureus sensitivity tests using the standard broth microdilution method" . "O, Z(MSM6046070901)" . "RIV/60460709:41340/14:63809!RIV15-MSM-41340___" . . "Thymoquinone vapor significantly affects the results of Staphylococcus aureus sensitivity tests using the standard broth microdilution method"@en . . "[70E00D544F3C]" . "RIV/60460709:41340/14:63809" . "Thymoquinone vapor significantly affects the results of Staphylococcus aureus sensitivity tests using the standard broth microdilution method" . "essential oil, microtiter plate, Nigella sativa, quinone, Staphylococcus aureus, volatile"@en . . . . "Fitoterapia" . "Kou\u0159imsk\u00E1, Lenka" . . . .