"Ry\u0161\u00E1nek, Pavel" . . "Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic" . "RIV/60460709:41210/09:31358" . "Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic" . "41210" . "5"^^ . "Prunus . PLMVd . HSVd . Multiplex RT-PCR; Stone fruit viroids"@en . "341399" . . . "Gom\u00E9z, G." . . "Myrta, Arben" . . "124" . "[EBE84269DAE0]" . . "Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic"@en . . . "European Journal of Plant Pathology" . "Hassan, Mohamed" . . . . "Pall\u00E1s, V." . . "0929-1873" . "Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic"@en . . "In this paper, we report a large-scale survey for the incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) in stone fruit collections and commercial orchards in the Czech Republic. From the 645 samples analysed, PLMVd was detected in 80 (26.6%) of peaches and the HSVd in 3 (1.3%) of apricot and 1 (0.33%) of peach trees. Sixty-seven accession of peach (44.6%) from the Czech Clonal GeneBank were infected by PLMVd. In addition, we used naturally infected trees to standardise the simultaneous detection of PLMVd and HSVd plus host mRNA as the control by means of one-step multiplex RTC-PCR. Eleven PLMVd and two HSVd isolates were sequenced and analysed. All the PLMVd variants were highly homologous (97\u2013100%) to previously reported PLMVd variants from Tunisian peach and almond trees, and clustered together in the previously reported phylogenetic group III. The HSVd variants obtained from apricot and peach trees were included in the previously proposed recombinant gr"@en . "RIV/60460709:41210/09:31358!RIV10-MZE-41210___" . "6"^^ . . "2"^^ . "In this paper, we report a large-scale survey for the incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) in stone fruit collections and commercial orchards in the Czech Republic. From the 645 samples analysed, PLMVd was detected in 80 (26.6%) of peaches and the HSVd in 3 (1.3%) of apricot and 1 (0.33%) of peach trees. Sixty-seven accession of peach (44.6%) from the Czech Clonal GeneBank were infected by PLMVd. In addition, we used naturally infected trees to standardise the simultaneous detection of PLMVd and HSVd plus host mRNA as the control by means of one-step multiplex RTC-PCR. Eleven PLMVd and two HSVd isolates were sequenced and analysed. All the PLMVd variants were highly homologous (97\u2013100%) to previously reported PLMVd variants from Tunisian peach and almond trees, and clustered together in the previously reported phylogenetic group III. The HSVd variants obtained from apricot and peach trees were included in the previously proposed recombinant gr" . "Hassan, Mohamed" . "P(QG60123)" . "2" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" .