. "41210" . "4"^^ . . "[BB236A3F3CAE]" . . . . "The objective of the study was to verify the hypothesis that GEN (genistein \u2013 phytoestrogen and an inhibitor of tyrosine protein kinase \u2013 TPK) effects on pig oocyte maturation and cumular cell expansion under in vitro conditions are connected with its estrogenic activity. Oocytes were cultivated for 24 hours up to the stage of the first meiotic metaphase (MI). Three different doses of GEN (13, 40, 80 \u00B5g/ml of medium) and also three doses of GIN, genistin, an analogue of GEN without effects on TPK, (80, 160 and 240 \u00B5g/ml of medium) were tested. To verify the reversibility of GEN effects, the oocytes were first cultivated for 24 hours with 80 \u00B5g of GEN per 1 ml of medium and then for another 24 hours without any GEN. GEN blocked pig oocyte maturation at the stage of the germinal vesicle (GV), depending on the dose. After rinsing out the GEN the oocyte maturation recovered, but with abnormalities (32%). GIN in a concentration of 80 \u00B5g/ml of medium induced a significant blockage at the GV stage" . "Effects of genistein and genistin on in vitro maturation of pig oocytes" . "Klabanov\u00E1, Petra" . . "CZ - \u010Cesk\u00E1 republika" . . "RIV/60460709:41210/08:25732" . . "Czech Journal of Animal Science" . . "5"^^ . "Effects of genistein and genistin on in vitro maturation of pig oocytes"@en . . "1212-1819" . . . . "Vodkov\u00E1, Zuzana" . "Effects of genistein and genistin on in vitro maturation of pig oocytes"@en . "53" . "RIV/60460709:41210/08:25732!RIV13-MSM-41210___" . "P(GD523/03/H076), Z(MSM6046070901), Z(MZE0002701401)" . "Petr, Jaroslav" . "1" . "365391" . . . . . "Effects of genistein and genistin on in vitro maturation of pig oocytes" . . . "Rajmon, Radko" . . "J\u00EDlek, Franti\u0161ek" . "8"^^ . . . . . "The objective of the study was to verify the hypothesis that GEN (genistein \u2013 phytoestrogen and an inhibitor of tyrosine protein kinase \u2013 TPK) effects on pig oocyte maturation and cumular cell expansion under in vitro conditions are connected with its estrogenic activity. Oocytes were cultivated for 24 hours up to the stage of the first meiotic metaphase (MI). Three different doses of GEN (13, 40, 80 \u00B5g/ml of medium) and also three doses of GIN, genistin, an analogue of GEN without effects on TPK, (80, 160 and 240 \u00B5g/ml of medium) were tested. To verify the reversibility of GEN effects, the oocytes were first cultivated for 24 hours with 80 \u00B5g of GEN per 1 ml of medium and then for another 24 hours without any GEN. GEN blocked pig oocyte maturation at the stage of the germinal vesicle (GV), depending on the dose. After rinsing out the GEN the oocyte maturation recovered, but with abnormalities (32%). GIN in a concentration of 80 \u00B5g/ml of medium induced a significant blockage at the GV stage"@en . "phytoestrogen, isoflavone, oocyte maturation, pig, estrogenic effect, tyrosine protein kinase"@en .