"Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L." . "426689" . "Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L."@en . . "RIV/60460709:41210/07:23442!RIV08-MSM-41210___" . "41210" . "Sofia, Bulgaria" . "Propagation of Ornamental Plants. Proc. of the 5th Intern. Conference \u201CPropagation of Ornamental Plants" . . . "Iniciace a histologie somatick\u00E9 embryogeneze a organogeneze u Phlox paniculata L."@cs . "SEJANI Publisher" . "Sofia, Bulgaria" . . "Matiska, Pavel" . "70;70" . "RIV/60460709:41210/07:23442" . . . . . "[BCCE5F760427]" . . "Iniciace a histologie somatick\u00E9 embryogeneze a organogeneze u Phlox paniculata L."@cs . . "Z(MSM6046070901)" . . "1"^^ . "Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L." . "Histological analysis of somatic embryogenesis and organogenesis derived from in vitro-grown leaves were assessed in Phlox paniculata L. Phlox cultivars \u00B4Fuji\u00B4 and \u00B4Starfire\u00B4 belong to important border perennials and are different in anthocyan occurrence. The leaf explants from sterile cultures were incubated on full-strenght MS salts (Murashige and Skoog, 1962) medium containing 0.5\u20131.0 mg.l-1 2,4-D (2,4-dichlorophenoxyacetic acid) with/without 0.2 mg.l-1 BA (benzyladenine). MS medium was supplemented with 30 or 90 g l-1 sucrose, B5 vitamins and 7.5 g l-1 agar Sigma. The intact leaf blades were cultured (abaxial side down) in the dark at temperature 22 +- 2 \u00B0C. The regeneration rate was expressed as the percentage of explant total number and the structures formed. The regeneration was highly depended on the genotype and concentration of 2,4-D and BA present in the culture medium. The significantly highest rate of regeneration (about 90 %) and callus induction were obtained on medium with 1 mg.l-1 2,4"@cs . . "2007-09-05+02:00"^^ . "978-954-90425-4-2" . "Neuvedeno, 0" . . "Histological analysis of somatic embryogenesis and organogenesis derived from in vitro-grown leaves were assessed in Phlox paniculata L. Phlox cultivars \u00B4Fuji\u00B4 and \u00B4Starfire\u00B4 belong to important border perennials and are different in anthocyan occurrence. The leaf explants from sterile cultures were incubated on full-strenght MS salts (Murashige and Skoog, 1962) medium containing 0.5\u20131.0 mg.l-1 2,4-D (2,4-dichlorophenoxyacetic acid) with/without 0.2 mg.l-1 BA (benzyladenine). MS medium was supplemented with 30 or 90 g l-1 sucrose, B5 vitamins and 7.5 g l-1 agar Sigma. The intact leaf blades were cultured (abaxial side down) in the dark at temperature 22 +- 2 \u00B0C. The regeneration rate was expressed as the percentage of explant total number and the structures formed. The regeneration was highly depended on the genotype and concentration of 2,4-D and BA present in the culture medium. The significantly highest rate of regeneration (about 90 %) and callus induction were obtained on medium with 1 mg.l-1 2,4"@en . . "1"^^ . "Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L."@en . "1"^^ . . "phlox paniculata, histology, somatic embryo, organogenesis"@en . "Histological analysis of somatic embryogenesis and organogenesis derived from in vitro-grown leaves were assessed in Phlox paniculata L. Phlox cultivars \u00B4Fuji\u00B4 and \u00B4Starfire\u00B4 belong to important border perennials and are different in anthocyan occurrence. The leaf explants from sterile cultures were incubated on full-strenght MS salts (Murashige and Skoog, 1962) medium containing 0.5\u20131.0 mg.l-1 2,4-D (2,4-dichlorophenoxyacetic acid) with/without 0.2 mg.l-1 BA (benzyladenine). MS medium was supplemented with 30 or 90 g l-1 sucrose, B5 vitamins and 7.5 g l-1 agar Sigma. The intact leaf blades were cultured (abaxial side down) in the dark at temperature 22 +- 2 \u00B0C. The regeneration rate was expressed as the percentage of explant total number and the structures formed. The regeneration was highly depended on the genotype and concentration of 2,4-D and BA present in the culture medium. The significantly highest rate of regeneration (about 90 %) and callus induction were obtained on medium with 1 mg.l-1 2,4" . . .