. "41210" . "There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called \u201Cageing\u201D, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time."@cs . . "Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)"@en . . . . "Rajmon, Radko" . "300;310" . "There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called \u201Cageing\u201D, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time."@en . "Czech Journal of Animal Science" . . . "Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)" . "[3A588C8604E9]" . . "5"^^ . . "RIV/60460709:41210/05:11703!RIV06-MSM-41210___" . "1212-1819" . "Zlep\u0161en\u00ED v\u00FDvojov\u00E9 kompetence st\u00E1rnouc\u00EDch oocyt\u016F po o\u0161et\u0159en\u00ED IGF1 a EGF"@cs . "CZ - \u010Cesk\u00E1 republika" . "There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called \u201Cageing\u201D, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time." . . . "6"^^ . "epidermal growth factor, insulin-like growth factor 1, oocyte, ageing, pig"@en . "50" . . "Z(MSM6046070901)" . "Petrov\u00E1, I." . "Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)"@en . "Sedm\u00EDkov\u00E1, Mark\u00E9ta" . "RIV/60460709:41210/05:11703" . . . "Rozinek, Ji\u0159\u00ED" . "J\u00EDlek, Franti\u0161ek" . . . "Kuthanov\u00E1, Zuzana" . . "7" . . . "Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)" . "524413" . . . "11"^^ . "Zlep\u0161en\u00ED v\u00FDvojov\u00E9 kompetence st\u00E1rnouc\u00EDch oocyt\u016F po o\u0161et\u0159en\u00ED IGF1 a EGF"@cs .