. . . . . . . "Z(MSM6046070901)" . "[8D8BF32FAD3A]" . . "2"^^ . "41210" . "33;33" . . "Identifikace faktor\u016F pathogenicity fytoparasitick\u00FDch nematod Ditylenchus dipsaci pomoc\u00ED cDNA-AFLP strategie"@cs . "Mendelova zem\u011Bd\u011Blsk\u00E1 a lesnick\u00E1 univerzita v Brn\u011B" . . . "80-7157-905-X" . "An Efficent CDNA-AFLP \u2013 Based Strategy for the Identification of Pathogenity Factors from the stem Nematode Ditylenchus dipsaci," . "2"^^ . "RIV/60460709:41210/05:11446" . . "Sborn\u00EDk abstrakt\u016F z konference poslucha\u010D\u016F postgradu\u00E1ln\u00EDho doktorsk\u00E9ho studia MendelNet 05 Agro, Mendelova zem\u011Bd\u011Blsk\u00E1 a lesnick\u00E1 univerzita v Brn\u011B" . "Ditylenchus dipsaci je voln\u011B \u017Eij\u00EDc\u00ED osn\u00ED fytoparasitick\u00FD nematod, kter\u00FD se vyskytuje v mnoha biologick\u00FDch ras\u00E1ch. Identifikace a funk\u010Dn\u00ED anal\u00FDza faktor\u016F pathogenicity produkovan\u00FDch fytoparasitick\u00FDmi nematody je nezbytn\u00FDm p\u0159edpokladem efektivn\u00EDho \u0161lecht\u011Bn\u00ED a transgenozy rostlin .Jednotliv\u00E9 v\u00FDvojov\u00E9 procesy v pr\u016Fb\u011Bhu ontogenese jsou na molekul\u00E1rn\u00ED \u00FArovni ur\u010Deny zm\u011Bnami v genov\u00E9 expresi. Jedn\u00EDm z efektivn\u00EDch p\u0159\u00EDstup\u016F ke studiu koordinovan\u00FDch transkriptom\u016F patogen-rostlina je strategie RNA \u201Efingerprintingu\u201C ozna\u010Dovan\u00E1 jako cDNA-AFLP. V na\u0161\u00ED pr\u00E1ci jsme pou\u017Eili tuto strategii pro charakterizaci transkriptomu D. dipsaci v pr\u016Fb\u011Bhu infek\u010Dn\u00EDho procesu. N\u00E1hodn\u011B bylo vybr\u00E1no \u0161est diferenci\u00E1ln\u011B exprimovan\u00FDch fragment\u016F, kter\u00E9 byly n\u00E1sledn\u011B klonov\u00E1ny a sekvenov\u00E1ny. Z\u00EDskan\u00E9 nukleotidov\u00E9 sekvence byly porovn\u00E1ny se sekvencemi v datab\u00E1zi GenBank pomoc\u00ED modulu BLASTX. Krom\u011B podobnosti s proteinov\u00FDmi sekvencemi Caenorhabditis elegans, jedna sekvence vykazuje vysokou m\u00EDru podobnosti s chitinasou Heterodera glycine. Na z\u00E1k"@cs . "2005-11-29+01:00"^^ . "RIV/60460709:41210/05:11446!RIV06-MSM-41210___" . "An Efficent CDNA-AFLP \u2013 Based Strategy for the Identification of Pathogenity Factors from the stem Nematode Ditylenchus dipsaci,"@en . "An Efficent CDNA-AFLP \u2013 Based Strategy for the Identification of Pathogenity Factors from the stem Nematode Ditylenchus dipsaci,"@en . . "Marek, Martin" . "Ry\u0161\u00E1nek, Pavel" . . "Identifikace faktor\u016F pathogenicity fytoparasitick\u00FDch nematod Ditylenchus dipsaci pomoc\u00ED cDNA-AFLP strategie"@cs . . . "Ditylenchus dipsaci, the stem nematode , is a migratory endoparasite of over 500 species of angiosperms. Biological responses and developmental processes are precisely controlled at the level of gene expression. Most pathogenicity factors are not expresse consitutively, but in highly coordinated way. A relatively simple method for profiling differential gene expression is cDNA-amplified fragment lenght polymorphism (cDNA-AFLP). We aimed to used the cDNA-AFLP technique to sample the D. dipsaci transcriptome and discovere genes specifically up-regulated during host infection. A random sample of six TDFs representating gene transcripts more abundant or specifically PCR amplified from infested plant tissues cDNAs, but not from non-infested plant material and nematode suspension cDNAs were cloned and sequenced after PCR re-amplification. Each sequence was compared by the BLASTX algorithm to sequences within the GenBank database. Besides similarities with Caenorhabditis elegans proteins, one TDF was signi"@en . . . . "Ditylenchus dipsaci, the stem nematode , is a migratory endoparasite of over 500 species of angiosperms. Biological responses and developmental processes are precisely controlled at the level of gene expression. Most pathogenicity factors are not expresse consitutively, but in highly coordinated way. A relatively simple method for profiling differential gene expression is cDNA-amplified fragment lenght polymorphism (cDNA-AFLP). We aimed to used the cDNA-AFLP technique to sample the D. dipsaci transcriptome and discovere genes specifically up-regulated during host infection. A random sample of six TDFs representating gene transcripts more abundant or specifically PCR amplified from infested plant tissues cDNAs, but not from non-infested plant material and nematode suspension cDNAs were cloned and sequenced after PCR re-amplification. Each sequence was compared by the BLASTX algorithm to sequences within the GenBank database. Besides similarities with Caenorhabditis elegans proteins, one TDF was signi" . . "Brno" . "Brno" . "1"^^ . "Neuvedeno, 0" . . "An Efficent CDNA-AFLP \u2013 Based Strategy for the Identification of Pathogenity Factors from the stem Nematode Ditylenchus dipsaci," . "511908" . "stem nematode, Ditylenchus dipsaci, differential gene expression, TDF-transcript-derived fragment, cDNA-AFLP, bioinformatic analyses, pathogenicity factors."@en .