. "V\u00FDvoj metody RT-PCR pro detekci viroidu latentn\u00ED mozaiky broskvon\u011B a viroidu zakrslosti chmele pro \u00FA\u010Dely certifikace sadbov\u00E9ho materi\u00E1lu"@cs . . . . "Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material." . "Zouhar, Miloslav" . "V\u00FDvoj metody RT-PCR pro detekci viroidu latentn\u00ED mozaiky broskvon\u011B a viroidu zakrslosti chmele pro \u00FA\u010Dely certifikace sadbov\u00E9ho materi\u00E1lu"@cs . . "RIV/60460709:41210/05:###12265" . . . "Acta Horticulturae" . "517737" . . "5" . "High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usingany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be detected in flower"@cs . "657" . . "Hassan, Mohamed Ahmed Ali" . . "Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material."@en . "Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material." . "PLMVd, HSVd, RNA extraction, RT-PCR, peach, certification"@en . "RIV/60460709:41210/05:###12265!RIV06-MZE-41210___" . "Ry\u0161\u00E1nek, Pavel" . "High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usingany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be detected in flower"@en . . "391;395" . "Hassan, Mohamed Ahmed Ali" . "Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material."@en . . "High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usingany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be detected in flower" . "41210" . . . . . "P(QC1359)" . "BE - Belgick\u00E9 kr\u00E1lovstv\u00ED" . . "0567-7572" . "5"^^ . "3"^^ . . "3"^^ . . "[68F07F301468]" .