"Biologick\u00E1 fakulta" . . . "The influence of biochanin A on the meiotic maturation of pig oocytes" . "pig, oocyte, meiosis, phytoestrogens, biochanin A"@en . . "Je\u0161eta, Michal" . "29;30" . . "Vliv biochaninu A na meiotick\u00E9 zr\u00E1n\u00ED oocyt\u016F prasete"@cs . "5"^^ . . . "5"^^ . "[9E5AF33BEBB7]" . "2"^^ . "Phytoestrogens are natural plant substances structurally similar to endogenous estrogens. There is evidence, that they influence the maturation of mammalian oocytes. Although biochanin A is one of the first phytoestrogens connected with reproductive disorders, there are no data about its influence on oocytes. The aim of our study was to verify biochanin A effects on the meiotic maturation of pig oocytes. Oocytes were cultivated for 24 hours to the MI stage of meiotic maturation. Various amounts of biochanin A (0, 20, 30, 40, 50, 60, 80 ug/ml) were added to the medium. Biochanin A inhibited pig oocyte maturation before germinal vesicle breakdown (GVBD) in a dose-dependent manner. The addition of 80 and 60 ug/ml of biochanin A already acted toxically."@en . "Vliv biochaninu A na meiotick\u00E9 zr\u00E1n\u00ED oocyt\u016F prasete"@cs . . . . . "Journal of Applied Biomedicine" . "Phytoestrogens are natural plant substances structurally similar to endogenous estrogens. There is evidence, that they influence the maturation of mammalian oocytes. Although biochanin A is one of the first phytoestrogens connected with reproductive disorders, there are no data about its influence on oocytes. The aim of our study was to verify biochanin A effects on the meiotic maturation of pig oocytes. Oocytes were cultivated for 24 hours to the MI stage of meiotic maturation. Various amounts of biochanin A (0, 20, 30, 40, 50, 60, 80 ug/ml) were added to the medium. Biochanin A inhibited pig oocyte maturation before germinal vesicle breakdown (GVBD) in a dose-dependent manner. The addition of 80 and 60 ug/ml of biochanin A already acted toxically." . "RIV/60460709:41210/05:###11694!RIV06-MZE-41210___" . . "524640" . . . "Kuthanov\u00E1, Zuzana" . "41210" . "The influence of biochanin A on the meiotic maturation of pig oocytes" . . "The influence of biochanin A on the meiotic maturation of pig oocytes"@en . . "Rajmon, Radko" . "Chmel\u00EDkov\u00E1, Eva" . . "\u010Cesk\u00E9 Bud\u011Bjovice" . . . "\u010Cesk\u00E9 Bud\u011Bjovice" . "2005-10-24+02:00"^^ . . . "Petr, Jaroslav" . "RIV/60460709:41210/05:###11694" . . "P(GD523/03/H076), P(QF3025), Z(MSM6046070901)" . . . "The influence of biochanin A on the meiotic maturation of pig oocytes"@en . "1214-0287" . . "Phytoestrogens are natural plant substances structurally similar to endogenous estrogens. There is evidence, that they influence the maturation of mammalian oocytes. Although biochanin A is one of the first phytoestrogens connected with reproductive disorders, there are no data about its influence on oocytes. The aim of our study was to verify biochanin A effects on the meiotic maturation of pig oocytes. Oocytes were cultivated for 24 hours to the MI stage of meiotic maturation. Various amounts of biochanin A (0, 20, 30, 40, 50, 60, 80 ug/ml) were added to the medium. Biochanin A inhibited pig oocyte maturation before germinal vesicle breakdown (GVBD) in a dose-dependent manner. The addition of 80 and 60 ug/ml of biochanin A already acted toxically."@cs . .