. . "3" . . . "10627" . "Kit, Yuriy" . . . "RIV/60162694:G44__/14:43875157" . . . . "Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings" . "Zastavna, Danuta" . . "Vajrychov\u00E1, Marie" . "55" . . . "Aim To develop and test a protocol for isolation of potential auto-antigens from chorionic tissue that may be linked to recurrent miscarriage (RM). Methods The strategy included: 1) isolation of IgGs tightly bound to chorionic tissue of RM patients by protein G chromatography; 2) construction of affinity columns using the chorionic antibodies for isolation of auto-antigens; 3) enrichment of auto-antigens from detergent extracted solution of chorionic proteins by affinity chromatography; 4) separation by dodecyl sulfate-electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry identification. Results Five potential auto-antigens were detected: neutral alpha-glucosidase AB, endoplasmin, transitional endoplasmic reticulum ATPase, putative endoplasmin-like protein, and cytoplasmic actin 2. Conclusions We developed a strategy for identification of auto-antigens in the chorionic tissue of women with RM, which could be of diagnostic and prognostic value."@en . "Starykovych, Marina" . . "Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings"@en . "G44" . . "6"^^ . "0353-9504" . . "Aim To develop and test a protocol for isolation of potential auto-antigens from chorionic tissue that may be linked to recurrent miscarriage (RM). Methods The strategy included: 1) isolation of IgGs tightly bound to chorionic tissue of RM patients by protein G chromatography; 2) construction of affinity columns using the chorionic antibodies for isolation of auto-antigens; 3) enrichment of auto-antigens from detergent extracted solution of chorionic proteins by affinity chromatography; 4) separation by dodecyl sulfate-electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry identification. Results Five potential auto-antigens were detected: neutral alpha-glucosidase AB, endoplasmin, transitional endoplasmic reticulum ATPase, putative endoplasmin-like protein, and cytoplasmic actin 2. Conclusions We developed a strategy for identification of auto-antigens in the chorionic tissue of women with RM, which could be of diagnostic and prognostic value." . "beta; women; disease; proteins; gp96; autoantibodies; pregnancy loss"@en . "Croatian Medical Journal" . . . "000338631600010" . "2"^^ . "Stoika, Rostyslav" . "Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings"@en . "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049207/" . "10.3325/cmj.2014.55.259" . . "RIV/60162694:G44__/14:43875157!RIV15-MO0-G44_____" . "[1053C3BDCB6B]" . "HR - Chorvatsk\u00E1 republika" . "N" . "Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings" . . "6"^^ . "Len\u010Do, Juraj" .