. "RIV/60162694:G44__/11:00002471!RIV12-MO0-G44_____" . "1214-021X" . . . . . "Pejchal, Jaroslav" . "IR; ionising radiation; ATM; ataxia telangiectasia mutated kinase; ATR; ATM- and Rad3-related protein kinase; chk-2; checkpoint kinase-2"@en . "In this study we used caffeine, a non-specific ATM inhibitor, and studied its effect on the activation of the proteins involved in cell cycle control and the induction of apoptosis in human T-lymphocyte leukaemic MOLT-4 cells (p53 wt) after irradiation. We evaluated the expression of the tumour-suppressor p53, the cell cycle regulator p21, and the anti-apoptotic protein myeloid cell leukemia 1 (Mcl-1). After treatment with 2 mM caffeine, the cells were irradiated by 1 or 3 Gy. The ATM/p53 pathway was suppressed, which led to increased apoptosis accompanied by a p53-independent decrease in Mcl-1. It also caused down-regulation of p21, which possibly contributed to the shortened cell cycle arrest necessary for effective DNA repair and thus impeded radio-resistance. Caffeine promotes the cytotoxic effect of ionising radiation and provides a possible platform for the development of new anti-cancer therapeutics known as radio-sensitizers." . "V\u00E1vrov\u00E1, Ji\u0159ina" . "http://dx.doi.org/10.2478/v10136-009-0031-7" . "Z\u00E1rybnick\u00E1, Lenka" . . . . . . "Tich\u00FD, Ale\u0161" . . . . "Muthn\u00E1, Darina" . "000284780800004" . "8"^^ . "G44" . "Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells"@en . "1" . "CZ - \u010Cesk\u00E1 republika" . . "Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells" . . . . "In this study we used caffeine, a non-specific ATM inhibitor, and studied its effect on the activation of the proteins involved in cell cycle control and the induction of apoptosis in human T-lymphocyte leukaemic MOLT-4 cells (p53 wt) after irradiation. We evaluated the expression of the tumour-suppressor p53, the cell cycle regulator p21, and the anti-apoptotic protein myeloid cell leukemia 1 (Mcl-1). After treatment with 2 mM caffeine, the cells were irradiated by 1 or 3 Gy. The ATM/p53 pathway was suppressed, which led to increased apoptosis accompanied by a p53-independent decrease in Mcl-1. It also caused down-regulation of p21, which possibly contributed to the shortened cell cycle arrest necessary for effective DNA repair and thus impeded radio-resistance. Caffeine promotes the cytotoxic effect of ionising radiation and provides a possible platform for the development of new anti-cancer therapeutics known as radio-sensitizers."@en . . "RIV/60162694:G44__/11:00002471" . . "Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells" . "9" . . "\u0158ez\u00E1\u010Dov\u00E1, Martina" . . "188999" . "Z(MO0FVZ0000501), Z(MSM0021620820)" . "5"^^ . "Journal of Applied Biomedicine - print" . . . . . "[81D41F987CCA]" . "\u0160inkorov\u00E1, Zuzana" . "7"^^ . "Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells"@en .