"Ku\u010Da, Kamil" . . "Bratislava Medical Journal-Bratislavske Lekarske Listy" . "Soukup, Ond\u0159ej" . . "In this work we aim to summarize the universality of this compound, its reactivatiom potency when different cholinesterase inhibitors are used. Background: Pralidoxime is considered as a gold standard of acetylcholinesterase reactivators - antidotes used in case of nerve agent poisonings. It is commercially available worldwide since many years back. However, several studies describe this oxime as an old-fashion antidote Methods: Pralidoxime was synthesized at our department. The reactivating efficacy was tested on 10% (w/v) rat brain homogenate that was incubated with appropriate inhibitor for 30 minutes to reach 96% inhibition of AChE. Then, pralidoxime was added for 10 minutes. Measurements were performed at 25 \u00B0C, pH 8, and at the concentrations of the AChE reactivators at 10-3 and 10-5 M. The activities of brain AChE were measured by a potentiostatic method. Results: No sufficient reactivation was achieved at the concentration 10-5 M, which is a concentration that is possible to reach after adm"@en . "Tobin, G." . . . "In this work we aim to summarize the universality of this compound, its reactivatiom potency when different cholinesterase inhibitors are used. Background: Pralidoxime is considered as a gold standard of acetylcholinesterase reactivators - antidotes used in case of nerve agent poisonings. It is commercially available worldwide since many years back. However, several studies describe this oxime as an old-fashion antidote Methods: Pralidoxime was synthesized at our department. The reactivating efficacy was tested on 10% (w/v) rat brain homogenate that was incubated with appropriate inhibitor for 30 minutes to reach 96% inhibition of AChE. Then, pralidoxime was added for 10 minutes. Measurements were performed at 25 \u00B0C, pH 8, and at the concentrations of the AChE reactivators at 10-3 and 10-5 M. The activities of brain AChE were measured by a potentiostatic method. Results: No sufficient reactivation was achieved at the concentration 10-5 M, which is a concentration that is possible to reach after adm" . "3"^^ . . "9" . "RIV/60162694:G44__/10:00002398" . . "111" . . "Hrabinov\u00E1, Martina" . "cholinesterase; reactivator; oxime; inhibitor; pralidoxime"@en . . "5"^^ . . "0006-9248" . . . "\u017D\u010F\u00E1rov\u00E1 Karasov\u00E1, Jana" . "6"^^ . "000283482300007" . . "280843" . "Pralidoxime - the gold standard of acetylcholinesterase reactivators - reactivation efficacy in vitro"@en . "G44" . . . . "[289AEB55EA02]" . . "Pralidoxime - the gold standard of acetylcholinesterase reactivators - reactivation efficacy in vitro" . . "Pralidoxime - the gold standard of acetylcholinesterase reactivators - reactivation efficacy in vitro" . . . . "SK - Slovensk\u00E1 republika" . . "Pohanka, Miroslav" . "P(ME 865)" . "Pralidoxime - the gold standard of acetylcholinesterase reactivators - reactivation efficacy in vitro"@en . "RIV/60162694:G44__/10:00002398!RIV11-MSM-G44_____" .