"The effect of trimedoxime on acetylcholinesterase and on the cholinergic system of the rat bladder"@en . "256227" . . "4"^^ . "The effect of trimedoxime on acetylcholinesterase and on the cholinergic system of the rat bladder" . "000277970800005" . "Holas, Ond\u0159ej" . "The effect of trimedoxime on acetylcholinesterase and on the cholinergic system of the rat bladder"@en . "RIV/60162694:G44__/10:00002293!RIV11-MO0-G44_____" . "G44" . . . "Jun, Daniel" . "Fusek, Josef" . "2010" . . "8"^^ . "[346142596D52]" . "Ku\u010Da, Kamil" . . . "The effect of trimedoxime on acetylcholinesterase and on the cholinergic system of the rat bladder" . "RIV/60162694:G44__/10:00002293" . . . "Killi, Kumar Uday" . . . "Journal of Applied Biomedicine - print" . . "I, Z(MO0FVZ0000604)" . "6"^^ . . . "8" . . . "1214-021X" . "acetylcholinesterase; trimedoxime; antidote; muscarinic receptors; reactivation"@en . "Tobin, Gunnar" . "We tested the affinity of tridoxime to acetylcholinesterase (AChE), its mechanism of interaction and effect on the cholinergic system of the rat bladder. The half maximal inhibitory concentration (IC50) of trimedoxime to recombinant AChE was found to be 82.0 mM +/- 30.1 mM, e.g weak inhibition. Its interaction with AChE seems to be very similar to obidoxime - one aromatic nucleus interacts with the peripheral anionic site and the other with the residues TYR337 and TYR341 inside the cavity. Also, oxime moiety is moving towards the catalytic triade ready for the reactivation of the inhibited AChE. In the organ bath experiment no significant effect of trimedoxime was observed on the contraction of the detrusor caused by the muscarinic agonist metacholine."@en . "We tested the affinity of tridoxime to acetylcholinesterase (AChE), its mechanism of interaction and effect on the cholinergic system of the rat bladder. The half maximal inhibitory concentration (IC50) of trimedoxime to recombinant AChE was found to be 82.0 mM +/- 30.1 mM, e.g weak inhibition. Its interaction with AChE seems to be very similar to obidoxime - one aromatic nucleus interacts with the peripheral anionic site and the other with the residues TYR337 and TYR341 inside the cavity. Also, oxime moiety is moving towards the catalytic triade ready for the reactivation of the inhibited AChE. In the organ bath experiment no significant effect of trimedoxime was observed on the contraction of the detrusor caused by the muscarinic agonist metacholine." . . . . . "CZ - \u010Cesk\u00E1 republika" . "Soukup, Ond\u0159ej" . "Binder, Ji\u0159\u00ED" . . .