"RP-HPLC ur\u010Den\u00ED lipofility bispyridiniov\u00FDch reaktiv\u00E1tor\u016F acetylcholinesterasy nesouc\u00EDch but-2-enov\u00FD spojovac\u00ED \u0159et\u011Bzec"@cs . "3"^^ . "391" . "Mus\u00EDlek, Kamil" . "Jamp\u00EDlek, Josef" . "acetylcholinesterase; reactivator; RP-HPLC; purity; . lipophilicity"@en . "000255185300035" . "RIV/60162694:G44__/08:00002001" . "Dohnal, Ji\u0159\u00ED" . "Dole\u017Eal, Mqrtin" . . . "6"^^ . . . . . "1618-2642" . . . . "Analytical and Bioanalytical Chemistry" . "Gunn-Moore, Frank" . . "RP-HPLC ur\u010Den\u00ED lipofility bispyridiniov\u00FDch reaktiv\u00E1tor\u016F acetylcholinesterasy nesouc\u00EDch but-2-enov\u00FD spojovac\u00ED \u0159et\u011Bzec"@cs . . . . . "393526" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . . "RP-HPLC lipophilicity determination of bispyridinium reactivators of acetylcholinesterase bearing a but-2-ene connecting linker" . "G44" . "RP-HPLC lipophilicity determination of bispyridinium reactivators of acetylcholinesterase bearing a but-2-ene connecting linker" . "P(GP305/07/P162), S, Z(MO0FVZ0000501)" . "[26EA214DC947]" . . "Jun, Daniel" . "7"^^ . "1" . "New acetylcholinesterase reactivators with either a (E) or (Z)-but-2-ene connecting linker were recently prepared. The purity of the compounds was checked by HPLC and was found to be sufficient for in-vitro screening. All the discussed bispyridinium reactivators were analyzed by reversed-phase high performance liquid chromatography (RP-HPLC) to measure lipophilicity. The procedure was performed under isocratic conditions with methanol as organic modifier in the mobile phase using an end-capped non-polar C18 stationary phase RP column. Relationships between the lipophilicity (logarithmof the RP-HPLC capacity factor, log k) and chemical structures of the studied compounds are discussed. Lipophilicity was different for the (E) and (Z) compounds and varied among the compounds in each of these groups. The lipophilicity differences also indicated an apparent influence of intramolecular interactions. Lipophilicity calculations (log P/Clog P) by means of commonly used software were not successful due to the p"@en . "Dvan\u00E1ct bispyridiniov\u00FDch slou\u010Denin nesouc\u00EDch (E)-but-2-enov\u00FD nebo (Z)-but-2-enov\u00FD spojovac\u00ED \u0159et\u011Bzec bylo testov\u00E1no pomoc\u00ED RP-HPLC metodiky na \u010Distotu a lipofilitu. Byly nalezeny d\u016Fle\u017Eit\u00E9 intramolekul\u00E1rn\u00ED interakce."@cs . "RP-HPLC lipophilicity determination of bispyridinium reactivators of acetylcholinesterase bearing a but-2-ene connecting linker"@en . "RIV/60162694:G44__/08:00002001!RIV09-MO0-G44_____" . . "Ku\u010Da, Kamil" . "RP-HPLC lipophilicity determination of bispyridinium reactivators of acetylcholinesterase bearing a but-2-ene connecting linker"@en . . . "New acetylcholinesterase reactivators with either a (E) or (Z)-but-2-ene connecting linker were recently prepared. The purity of the compounds was checked by HPLC and was found to be sufficient for in-vitro screening. All the discussed bispyridinium reactivators were analyzed by reversed-phase high performance liquid chromatography (RP-HPLC) to measure lipophilicity. The procedure was performed under isocratic conditions with methanol as organic modifier in the mobile phase using an end-capped non-polar C18 stationary phase RP column. Relationships between the lipophilicity (logarithmof the RP-HPLC capacity factor, log k) and chemical structures of the studied compounds are discussed. Lipophilicity was different for the (E) and (Z) compounds and varied among the compounds in each of these groups. The lipophilicity differences also indicated an apparent influence of intramolecular interactions. Lipophilicity calculations (log P/Clog P) by means of commonly used software were not successful due to the p" . . .