"G44" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "P(OPUOFVZ200603)" . . "Potency of several oximes to reactivate human acetylcholinesterase and butyrylcholinesterase inhibited by paraoxon in vitro" . "Schopnost vybran\u00FDch oxim\u016F reaktivovat lidskou acetylcholinester\u00E1zu a butyrylcholinester\u00E1zu inhibovanou paraoxonem in vitro"@cs . "Organophosphorus pesticides (e.g. chlorpyrifos, malathion, and parathion) and nerve agents (sarin, tabun, and VX) are highly toxic organophosphorus compounds with strong inhibition potency against two key enzymes in the human body-acetylcholinesterase (AChE; EC 3.1.1.7) and butyrylcholinesterase (BuChE; EC 3.1.1.8). Subsequent accumulation of acetylcholine at synaptic clefts can result in cholinergic crisis and possible death of intoxicated organism. For the recovery of inhibited AChE, derivatives from the group of pyridinium or bispyridinium aldoximes (called oximes) are used. Their efficacy depends on their chemical structure and also type of organophosphorus inhibitor. In this study, we have tested potency of selected cholinesterase reactivators (pralidoxime, obidoxime, trimedoxime, methoxime and H-oxime HI-6) to reactivate human erythrocyte AChE and human plasma BuChE inhibited by pesticide paraoxon. For this purpose, modified Ellman's method was used and two different concentrations of oxime" . "P\u00EDcha, Jan" . . . "RIV/60162694:G44__/08:00001983" . "Potency of several oximes to reactivate human acetylcholinesterase and butyrylcholinesterase inhibited by paraoxon in vitro"@en . "[9DECD2F52EB5]" . . . . . . . . "RIV/60162694:G44__/08:00001983!RIV09-MO0-G44_____" . "Organophosphorus pesticides (e.g. chlorpyrifos, malathion, and parathion) and nerve agents (sarin, tabun, and VX) are highly toxic organophosphorus compounds with strong inhibition potency against two key enzymes in the human body-acetylcholinesterase (AChE; EC 3.1.1.7) and butyrylcholinesterase (BuChE; EC 3.1.1.8). Subsequent accumulation of acetylcholine at synaptic clefts can result in cholinergic crisis and possible death of intoxicated organism. For the recovery of inhibited AChE, derivatives from the group of pyridinium or bispyridinium aldoximes (called oximes) are used. Their efficacy depends on their chemical structure and also type of organophosphorus inhibitor. In this study, we have tested potency of selected cholinesterase reactivators (pralidoxime, obidoxime, trimedoxime, methoxime and H-oxime HI-6) to reactivate human erythrocyte AChE and human plasma BuChE inhibited by pesticide paraoxon. For this purpose, modified Ellman's method was used and two different concentrations of oxime"@en . "Marek, Jan" . "Drug and Chemical Toxicology" . "Ku\u010Da, Kamil" . "31" . . . "000252323200001" . . "5"^^ . . "Organofosforov\u00E9 pesticidy (nap\u0159. chlorpyrifos, malathion a parathion) a nervov\u011B paralytick\u00E9 l\u00E1tky (sarin, tabun a VX) jsou vysoce toxick\u00E9 organofosf\u00E1ty s vysokou inhibi\u010Dn\u00ED schopnost\u00ED v\u016F\u010Di dv\u011Bma kl\u00ED\u010Dov\u00FDm enzym\u016Fm v lidsk\u00E9m t\u011Ble - acetylcholinester\u00E1ze (AChE; EC 3.1.1.7) a butyrylcholinester\u00E1ze (BuChE; EC 3.1.1.8). N\u00E1sledn\u00E9 nahromad\u011Bn\u00ED acetylcholinu v synaptick\u00FDch \u0161t\u011Brbin\u00E1ch m\u016F\u017Ee vy\u00FAstit v cholinergn\u00ED krizi a smrt intoxikovan\u00E9ho organizmu. Pro obnovu aktivity inhibovan\u00E9 AChE jsou u\u017E\u00EDv\u00E1ny deriv\u00E1ty ze skupiny pyridinov\u00FDch a bispyridiniov\u00FDch reaktiv\u00E1tor\u016F (naz\u00FDvan\u00E9 oximy). Jejich \u00FA\u010Dinnost z\u00E1vis\u00ED na jejich chemick\u00E9 struktu\u0159e a struktu\u0159e organofosforov\u00E9ho inhibitoru. V t\u00E9to studii jsme otestovali schopnost vybran\u00FDch reaktiv\u00E1tor\u016F cholinester\u00E1z (pralidoxim, obidoxim, trimedoxim, methoxim a H-oxim HI-6) reaktivovat lidskou erytrocyt\u00E1rn\u00ED AChE a plazmatickou BuChE inhibovanou pesticidem paraoxon. Pro testov\u00E1n\u00ED byla pou\u017Eita modifikovan\u00E1 metoda podle Ellmana, l\u00E1tky byly m\u011B\u0159eny ve dvou koncentrac\u00EDch (10 a 100 \u03BCM)"@cs . "acetylcholinesterase; butyrylcholinesterase; pesticide; organophosphate; reactivator; oxime; bioscavenger"@en . "0148-0545" . "Potency of several oximes to reactivate human acetylcholinesterase and butyrylcholinesterase inhibited by paraoxon in vitro" . . "Schopnost vybran\u00FDch oxim\u016F reaktivovat lidskou acetylcholinester\u00E1zu a butyrylcholinester\u00E1zu inhibovanou paraoxonem in vitro"@cs . "3"^^ . . . . "9"^^ . "388082" . "Kole\u010Dk\u00E1\u0159, V\u00EDt" . "1" . . . "Potency of several oximes to reactivate human acetylcholinesterase and butyrylcholinesterase inhibited by paraoxon in vitro"@en . "Jun, Daniel" . .