"An integrated mass spectrometric and computational framework for the analysis of protein interaction networks"@en . "Aebersold, Ruedi" . "An integrated mass spectrometric and computational framework for the analysis of protein interaction networks"@en . "Gstaiger, Matthias" . "6"^^ . . "Biologick\u00E9 syst\u00E9my jsou kontrolov\u00E1ny proteinov\u00FDmi komplexy, kter\u00E9 vytv\u00E1\u0159ej\u00ED dynamickou interak\u010Dn\u00ED s\u00ED\u0165. Popisujeme strategii, kter\u00E1 je schopn\u00E1 analyzovat proteinov\u00E9 komplexy pomoc\u00ED propojen\u00ED kvantitavivn\u00ED hmotnostn\u00ED spektrometrie bez pou\u017Eit\u00ED izotopick\u00FDch zna\u010Dek a po\u010D\u00EDta\u010Dov\u00E9 anal\u00FDzy. Porovn\u00E1n\u00EDm intensity peptidov\u00FDch profil\u016F mezi vzorky s postupn\u00FDm \u0159ed\u011Bn\u00EDm, tzv. MasterMap syst\u00E9m je schopen identifikovat specificky interaguj\u00EDc\u00ED proteiny, zaznamenat zm\u011Bny ve slo\u017Een\u00ED proteinov\u00FDch komplex\u016F a odhalit variace ve stavu fosforylace jednotliv\u00FDch komponent. Pou\u017Eili jsme komplex obsahuj\u00EDc\u00ED lidsk\u00FD transkrip\u010Dn\u00ED faktor FoxO3A, abychom dolo\u017Eili spolehlivost a pou\u017Eitelnost t\u00E9to technologie. Identifikovali jsme zn\u00E1m\u00E9 i nezn\u00E1m\u00E9 interakce FoxO3A s 14-3-3 proteiny, nav\u00EDc jsme identifikovali fosforyla\u010Dn\u00ED m\u00EDsta FoxO3A a detekovali nav\u00EDzanou redukovanou formu 14-3-3 po inhibici fosfoinositide-3 kin\u00E1zou. MasterMap syst\u00E9m posouv\u00E1 n\u011Bkolik limituj\u00EDc\u00EDch faktor\u016F sou\u010Dasn\u00FDch p\u0159\u00EDstup\u016F anal\u00FDzy proteinov\u00FDch komplex\u016F pomoc\u00ED lep\u0161\u00ED specific"@cs . . "3" . . "RIV/60162694:G44__/07:00001756!RIV08-MO0-G44_____" . "Rinner, Oliver" . . "Biological systems are controlled by protein complexes that associate into dynamic protein interaction networks. We describe a strategy that analyzes protein complexes through the integration of label-free, quantitative mass spectrometry and computational analysis. By evaluating peptide intensity profiles throughout the sequential dilution of samples, the MasterMap system identifies specific interaction partners, detects changes in the composition of protein complexes and reveals variations in the phosphorylation states of components of protein complexes. We use the complexes containing the human forkhead transcription factor FoxO3A to demonstrate the validity and performance of this technology. Our analysis identifies previously known and unknown interactions of FoxO3A with 14-3-3 proteins, in addition to identifying FoxO3A phosphorylation sites and detecting reduced 14-3-3 binding following inhibition of phosphoinositide-3 kinase. By improving specificity and sensitivity of interaction networks, ass" . . . . "Muller, Marcus" . . "An integrated mass spectrometric and computational framework for the analysis of protein interaction networks" . . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "Biological systems are controlled by protein complexes that associate into dynamic protein interaction networks. We describe a strategy that analyzes protein complexes through the integration of label-free, quantitative mass spectrometry and computational analysis. By evaluating peptide intensity profiles throughout the sequential dilution of samples, the MasterMap system identifies specific interaction partners, detects changes in the composition of protein complexes and reveals variations in the phosphorylation states of components of protein complexes. We use the complexes containing the human forkhead transcription factor FoxO3A to demonstrate the validity and performance of this technology. Our analysis identifies previously known and unknown interactions of FoxO3A with 14-3-3 proteins, in addition to identifying FoxO3A phosphorylation sites and detecting reduced 14-3-3 binding following inhibition of phosphoinositide-3 kinase. By improving specificity and sensitivity of interaction networks, ass"@en . . . "Integrovan\u00FD p\u0159\u00EDstup kombinuj\u00EDc\u00ED hmotnostn\u00ED spektrometrii a v\u00FDpo\u010Dty pro anal\u00FDzu proteinov\u00FDch interak\u010Dn\u00EDch s\u00EDt\u00ED"@cs . "G44" . "409341" . . . . "8"^^ . "25" . "Nature Biotechnology" . "P(OPUOFVZ200602)" . . "1087-0156" . "RIV/60162694:G44__/07:00001756" . "M\u00FCller, Lucas N." . . "Hub\u00E1lek, Martin" . . "1"^^ . "Integrovan\u00FD p\u0159\u00EDstup kombinuj\u00EDc\u00ED hmotnostn\u00ED spektrometrii a v\u00FDpo\u010Dty pro anal\u00FDzu proteinov\u00FDch interak\u010Dn\u00EDch s\u00EDt\u00ED"@cs . . . . "An integrated mass spectrometric and computational framework for the analysis of protein interaction networks" . "[971BD4B5DFFB]" . "14-3-3 Proteins; Algorithms; Chromatography, Liquid; Forkhead Transcription Factors; Internet; Mass Spectrometry; Phosphorylation; Protein Interaction Mapping; Protein Processing, Post-Translational; Proteome; Proteomics; Sensitivity and Specificity"@en . . "345-352" . . .