"Tularaemia caused by inhalation of type A Francisella tularensis bacteria is one of the most aggressive infectious diseases known, but the reasons for the very rapid spread of the organism from the lungs to internal organs and the ensuingmortality are unknown. The present study used the mouse model to examine in detail the host immune response in the lung. After an aerosol challenge with 20 c.f.u. of the type A strain FSC033, all mice developed clinical signs of severe disease, showed weight loss by day 4 of infection and died the next day. Histopathological findings in the lung revealed acute inflammation and intense vasculitis and perivasculitis on day 4. Gene transcriptional changes in the mouse lung samples were examined on days 1, 2 and 4 of infection using a cDNA microarray with 20,600 mouse clones representing 18,500 genes. In total, 424 genes were found to be differentially expressed, some of which were both up- and downregulated at different time points, 192 of which were upregulated and 234" . "Transcriptional profiling of host responses in mouse lungs following aerosol infection with type A Francisella tularensis"@en . "Transcriptional profiling of host responses in mouse lungs following aerosol infection with type A Francisella tularensis" . . . . "Chen, Wangxue" . "Ryden, Patrik" . . . "[439CA2630F20]" . . "Hartmanov\u00E1, Blanka" . "G44" . "Z(MO0FVZ0000501)" . "0022-2615" . "Transkrip\u010Dn\u00ED profil odpov\u011Bdi hostitele v my\u0161\u00EDch plic\u00EDch po aerosolov\u00E9 infekci typem A Francisella tularensis"@cs . "Andersson, Henrik" . . . "263-271" . "RIV/60162694:G44__/06:00001441" . "Transcriptional profiling of host responses in mouse lungs following aerosol infection with type A Francisella tularensis" . "Journal of Medical Microbiology" . "Tularaemia caused by inhalation of type A Francisella tularensis bacteria is one of the most aggressive infectious diseases known, but the reasons for the very rapid spread of the organism from the lungs to internal organs and the ensuingmortality are unknown. The present study used the mouse model to examine in detail the host immune response in the lung. After an aerosol challenge with 20 c.f.u. of the type A strain FSC033, all mice developed clinical signs of severe disease, showed weight loss by day 4 of infection and died the next day. Histopathological findings in the lung revealed acute inflammation and intense vasculitis and perivasculitis on day 4. Gene transcriptional changes in the mouse lung samples were examined on days 1, 2 and 4 of infection using a cDNA microarray with 20,600 mouse clones representing 18,500 genes. In total, 424 genes were found to be differentially expressed, some of which were both up- and downregulated at different time points, 192 of which were upregulated and 234"@en . "Conlan, Wayne" . . "Tular\u00E9mie vyvolan\u00E1 inhalac\u00ED typem A Francisella tularensis je jednou z nejagresivn\u011Bj\u0161\u00EDch, zn\u00E1m\u00FDch onemocn\u011Bn\u00ED, nicm\u00E9n\u011B p\u0159\u00ED\u010Diny rychl\u00E9ho \u0161\u00ED\u0159en\u00ED mikrob\u016F z plic do vnit\u0159n\u00EDch org\u00E1n\u016F a z toho vypl\u00FDvaj\u00EDc\u00ED mortalita jsou nezn\u00E1m\u00E9. V p\u0159edlo\u017Een\u00E9 studii byl pou\u017Eit my\u0161\u00ED model pro anal\u00FDzu hostitelsk\u00E9 imunitn\u00ED reakce v plic\u00EDch. Aplikace aerosolov\u00E9 infekce v d\u00E1vce 20 c.f.u. subtypem A kmen FSC033 vedla u v\u0161ech my\u0161\u00ED k v\u00FDvoji klinick\u00FDch znak\u016F z\u00E1va\u017En\u00E9ho onemocn\u011Bn\u00ED. V\u0161echny my\u0161i vykazovaly v\u00E1hov\u00FD \u00FAbytek 4. den po infekci a n\u00E1sleduj\u00EDc\u00ED den zem\u0159ely. Histopatologick\u00FD n\u00E1lez v plic\u00EDch 4. den po infekci prok\u00E1zal akutn\u00ED z\u00E1n\u011Bt a intenzivn\u00ED vaskulitidu a perivaskulitidu. Zm\u011Bny v genov\u00E9 transkripci v my\u0161\u00EDch plic\u00EDch byly analyzov\u00E1ny 1., 2. a 4. den po infekci pomoc\u00ED cDNA microarray obsahuj\u00EDc\u00EDch 20,600 my\u0161\u00EDch klon\u016F reprezentuj\u00EDc\u00EDch 18,500 gen\u016F. Celkov\u011B byla prok\u00E1z\u00E1na zm\u011Bna exprese u 424 gen\u016F v r\u016Fzn\u00FDch \u010Dasov\u00FDch intervalech, z nich\u017E n\u011Bkter\u00E9 geny byly p\u0159episov\u00E1ny ve zv\u00FD\u0161en\u00E9 m\u00ED\u0159e, u jin\u00FDch pak byla transkripce zeslabena. Bylo zji\u0161t\u011Bno,"@cs . "9"^^ . . "1"^^ . "3" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Sjostedt, Anders" . "Kuolee, Rhonda" . "Transkrip\u010Dn\u00ED profil odpov\u011Bdi hostitele v my\u0161\u00EDch plic\u00EDch po aerosolov\u00E9 infekci typem A Francisella tularensis"@cs . "13"^^ . . . "RIV/60162694:G44__/06:00001441!RIV07-MO0-G44_____" . "Transcriptional profiling of host responses in mouse lungs following aerosol infection with type A Francisella tularensis"@en . . "504157" . . "Francisella tularensis; aerosol infection; transcriptom; lungs"@en . "55" . . .