"Francisella tularensis live vaccine strain infection of mice has been established as an experimental model of tularemia that is suitable for studies of immune mechanisms against the intracellular pathogen. In this study, the model was used to explore immunogenic repertoire of F. tularensis with the aim of identifying new molecules able to activate the host immune system, potential bacterial markers with vaccine, and diagnostic applications. Immunoproteomic approach based on the combination of two-dimensional gel electrophoresis, immunoblotting, and mass spectrometry was applied. Globally, 36 different proteins were identified, which strongly reacted with sera from experimentally infected mice, including several putative virulence markers of intracellular pathogens as nucleoside diphosphate kinase, isocitrate dehydrogenase, RNA-binding protein Hfq, and molecular chaperone ClpB. Of them, 27 proteins are described for the first time as immunorelevant Francisella proteins. When comparing murine immunoprot"@en . "10"^^ . . . "Proteomic analysis of anti-Francisella tularensis LVS antibody response in murine model of tularemia"@en . . "P(OBVLAJEP20033), Z(MO0990200X001)" . "Proteomov\u00E1 anal\u00FDza anti-Francisella tularensis LVS protil\u00E1tkov\u00E9 odezvy v my\u0161\u00EDm modeluf tular\u00E9mie"@cs . "Proteomics" . "8" . . "Francisella tularensis live vaccine strain infection of mice has been established as an experimental model of tularemia that is suitable for studies of immune mechanisms against the intracellular pathogen. In this study, the model was used to explore immunogenic repertoire of F. tularensis with the aim of identifying new molecules able to activate the host immune system, potential bacterial markers with vaccine, and diagnostic applications. Immunoproteomic approach based on the combination of two-dimensional gel electrophoresis, immunoblotting, and mass spectrometry was applied. Globally, 36 different proteins were identified, which strongly reacted with sera from experimentally infected mice, including several putative virulence markers of intracellular pathogens as nucleoside diphosphate kinase, isocitrate dehydrogenase, RNA-binding protein Hfq, and molecular chaperone ClpB. Of them, 27 proteins are described for the first time as immunorelevant Francisella proteins. When comparing murine immunoprot" . "Proteomic analysis of anti-Francisella tularensis LVS antibody response in murine model of tularemia" . "Brychta, M." . . "Hernychov\u00E1, Lenka" . . "Proteomic analysis of anti-Francisella tularensis LVS antibody response in murine model of tularemia" . "Francisella tularensis; murine model; proteomic analysis"@en . "Proteomov\u00E1 anal\u00FDza anti-Francisella tularensis LVS protil\u00E1tkov\u00E9 odezvy v my\u0161\u00EDm modeluf tular\u00E9mie"@cs . "Kro\u010Dov\u00E1, Zuzana" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "539387" . "Len\u010Do, Juraj" . "Stul\u00EDk, Ji\u0159\u00ED" . "Proteomov\u00E1 anal\u00FDza anti-Francisella tularensis LVS protil\u00E1tkov\u00E9 odezvy v my\u0161\u00EDm modeluf tular\u00E9mie."@cs . "RIV/60162694:G44__/05:00001271" . . . "RIV/60162694:G44__/05:00001271!RIV06-MO0-G44_____" . . "1615-9853" . . . "Lundquist, M." . "G44" . . "Proteomic analysis of anti-Francisella tularensis LVS antibody response in murine model of tularemia"@en . . "2090-2103" . . "Hub\u00E1lek, Martin" . . . "[64E025E26C22]" . "5" . . . . "Larsson, P." . . "Macela, Ale\u0161" . "6"^^ . "14"^^ . . . "Havlasov\u00E1, J." .