. "4"^^ . . "Byla studov\u00E1na \u00FA\u010Dinnost reaktiv\u00E1tor\u016F 2-PAM, TMB-4, HI-6 a Toxogoninu p\u0159i reaktivaci acetylcholinester\u00E1zy inhibovan\u00E9 l\u00E1tkou R-33. Ke studiu byla vyu\u017Eita acetylcholinester\u00E1za imobilizovan\u00E1 a stabilizovan\u00E1 na bavln\u011Bn\u00E9 textilii a indika\u010Dn\u00ED pap\u00EDr s acetylthiocholin jodidem a kyselinou 5,5\u00B4-dithiobis(2-nitrobenzoovou). Aktivita enzymu byla zji\u0161\u0165ov\u00E1na m\u011B\u0159en\u00EDm remisn\u00EDch zm\u011Bn povrchu textilie, kter\u00E9 jsou zp\u016Fsoben\u00E9 reakc\u00ED produkt\u016F enzymov\u00E9 hydrol\u00FDzy substr\u00E1tu s chromogenn\u00EDm \u010Dinidlem. Nej\u00FA\u010Dinn\u011Bj\u0161\u00EDm ze studovan\u00FDch reaktiv\u00E1tor\u016F byl 2-PAM v koncentrac\u00EDch 0,5 mg.cm-3 a 0,1 mg.cm-3. HI-6 byl v t\u011Bchto koncentrac\u00EDch m\u00E9n\u011B \u00FA\u010Dinn\u00FD. Vy\u0161\u0161\u00ED \u00FA\u010Dinnost byla zaznamen\u00E1 a\u017E po 20 min v koncentraci 0,05 mg.cm-3 a 0,01 mg.cm-3, kde p\u0159evy\u0161ovala 2-PAM. U TMB-4 a Toxogoninu byly po 10 min zaznamenan\u00E9 hodnoty reaktivace kolem 10 %, po 20 min byly tyto reaktiv\u00E1tory ne\u00FA\u010Dinn\u00E9."@cs . . "Efficacy of 2-PAM, TMB-4, HI-6 and toxogonin in reactivation of immobilized acetylcholinesterase inhibited by compound R-33"@en . "Efficacy of 2-PAM, TMB-4, HI-6 and toxogonin in reactivation of immobilized acetylcholinesterase inhibited by compound R-33" . "RIV/60162694:G42__/07:#0000338" . . "Z(MO0UOPZHN0401)" . "Acetylcholinesterasa, inhibition, Vx, R-33, reactivation, 2-PAM, TMB-4, HI-6, Toxogonin"@en . "G42" . . . "[4A8D2971214C]" . . . "Kobliha, Zbyn\u011Bk" . "17" . "- Efficacy of reactivators 2-PAM, TMB-4, HI-6 and toxogonin in reactivation of acetylcholinesterase inhibited by compound R 33 has been studied. The study was performed with acetylcholinesterase immobilized and stabilized on a cotton fabric and with an indicator paper with acetylthiocholine iodide and 5,5\u00B4 dithiobis(2 nitrobenzoic acid). The enzyme activity was determined by measurement of remission changes of the fabric surface, caused by reaction of the chromogenic reagent with products of enzymatic hydrolysis of the substrate. The most potent of the reactivators studied was 2-PAM at concentrations 0.5 mg.cm-3 and 0.1 mg.cm-3. Effectivity of this reactivator was over 20 %. At these concentrations, reactivator HI-6 was less effective. Efficacy higher than that of 2-PAM was observed only after 20 min at concentrations 0.05 mg.cm-3 and 0.01 mg.cm-3. For TMB-4 and toxogonin the reactivation values were about 10 % after 10 min whereas after 15 or 20 min these reactivators were ineffective." . "\u00DA\u010Dinnost 2-PAM, TMB-4, HI-6 a Toxogoninu p\u0159i reaktivaci imobilizovan\u00E9 acetylcholinester\u00E1zy inhibovan\u00E9 l\u00E1tkou R-33"@cs . "Hal\u00E1mek, Emil" . "Efficacy of 2-PAM, TMB-4, HI-6 and toxogonin in reactivation of immobilized acetylcholinesterase inhibited by compound R-33"@en . . . . "- Efficacy of reactivators 2-PAM, TMB-4, HI-6 and toxogonin in reactivation of acetylcholinesterase inhibited by compound R 33 has been studied. The study was performed with acetylcholinesterase immobilized and stabilized on a cotton fabric and with an indicator paper with acetylthiocholine iodide and 5,5\u00B4 dithiobis(2 nitrobenzoic acid). The enzyme activity was determined by measurement of remission changes of the fabric surface, caused by reaction of the chromogenic reagent with products of enzymatic hydrolysis of the substrate. The most potent of the reactivators studied was 2-PAM at concentrations 0.5 mg.cm-3 and 0.1 mg.cm-3. Effectivity of this reactivator was over 20 %. At these concentrations, reactivator HI-6 was less effective. Efficacy higher than that of 2-PAM was observed only after 20 min at concentrations 0.05 mg.cm-3 and 0.01 mg.cm-3. For TMB-4 and toxogonin the reactivation values were about 10 % after 10 min whereas after 15 or 20 min these reactivators were ineffective."@en . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "RIV/60162694:G42__/07:#0000338!RIV07-MO0-G42_____" . "3"^^ . . "3" . . . . "4"^^ . "Tu\u0161arov\u00E1, Ivana" . "161-164" . "Hoskovcov\u00E1, Monika" . "\u00DA\u010Dinnost 2-PAM, TMB-4, HI-6 a Toxogoninu p\u0159i reaktivaci imobilizovan\u00E9 acetylcholinester\u00E1zy inhibovan\u00E9 l\u00E1tkou R-33"@cs . . "1537-6524" . . . "Efficacy of 2-PAM, TMB-4, HI-6 and toxogonin in reactivation of immobilized acetylcholinesterase inhibited by compound R-33" . . "Toxicology Mechanisms and Methods" . . "419333" . . .