. . . "Moravec, Tom\u00E1\u0161" . "978-80-7375-234-7" . . "Plchov\u00E1, Helena" . . "2008-09-09+02:00"^^ . . . "4"^^ . . "[0C373BBB4C4D]" . "PVX; recombinant antibodies; laboratory diagnozis"@en . "K\u0159tiny" . "PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST RECOMBINANT POTATO VIRUS X COAT PROTEIN"@en . "5"^^ . "Filigarov\u00E1, Marie" . "RIV/60109807:_____/08:0R000676!RIV13-MSM-60109807" . . "PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST RECOMBINANT POTATO VIRUS X COAT PROTEIN"@en . "PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST RECOMBINANT POTATO VIRUS X COAT PROTEIN" . . . "\u010Ce\u0159ovsk\u00E1, Noemi" . "Neuveden" . "1"^^ . "390089" . "The gene encoding the coat protein (CP) of Potato virus X was cloned into expression vector pMPM-4A Omega. PVX CP was expressed in Escherichia coli and was used for raising rabbit polyclonal antibodies. For immunization we purified CP fractions from bacterial lysate either by simple fractionation or by excision from SDS gel. The obtained sera and antibodies were tested for the detection of PVX laboratory hosts Nicotiana tabacum, cv. Petit Havana SR 1 and N.benthamiana and in various cultivars of natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies were specific for PVX and are usable for the detection of the broad isolates spectrum of the Potato virus X by ELISA and Western blot."@en . . "PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST RECOMBINANT POTATO VIRUS X COAT PROTEIN" . . . "P(1M06030), Z(AV0Z50380511)" . . . "Funk\u010Dn\u00ED genomika a proteomika ve \u0161lecht\u011Bn\u00ED rostlin" . "The gene encoding the coat protein (CP) of Potato virus X was cloned into expression vector pMPM-4A Omega. PVX CP was expressed in Escherichia coli and was used for raising rabbit polyclonal antibodies. For immunization we purified CP fractions from bacterial lysate either by simple fractionation or by excision from SDS gel. The obtained sera and antibodies were tested for the detection of PVX laboratory hosts Nicotiana tabacum, cv. Petit Havana SR 1 and N.benthamiana and in various cultivars of natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies were specific for PVX and are usable for the detection of the broad isolates spectrum of the Potato virus X by ELISA and Western blot." . "Brno" . "RIV/60109807:_____/08:0R000676" . . "D\u011Bdi\u010D, Petr" . .