"9"^^ . "V\u011Bdeck\u00E9 pr\u00E1ce" . . "Shortening of time of the plant regeneration from protoplast-derived calli in genus Solanum" . "Zkr\u00E1cen\u00ED doby regenerace rostlin na kalusech protoplastov\u00E9ho p\u016Fvodu u rodu Solanum"@cs . "Protoplast yield from plants cultivated on MS medium were compared with SH medium. Protoplast yield from plants cultivated on SH medium was unambiguously better in case when donor plants were maintained and subcultured on the medium in the stage of intensive growth.The protoplast-derived calli were tested for response to the graded contents of the plant hormones with ratio 1:5 IAA:ZT and to the contents with different ratios from 1:4 to 1:25 IAA and ZT with aim to short time needed for organogenesis. The experiments were performed in tissue-culture plates with 4 x 6 wells. The application period was 90 days. The perspective combination was 0.1 mg/1 IAA, 2 mg/l ZT. The necessary time for start of shoot regeneration was in average 1 month shorter as compared to earlier experience. The positive effect was observed especially on protoplast-derived calli after electrofusion. A gradually increased concentration of growth regulators with the ratio 1:5 auxin:cytokinin did not result in plant regeneration." . . "protoplast-derived calli; Solanum tuberosum; wild Solanum species; plant regeneration; plant hormones"@en . . "Protoplast yield from plants cultivated on MS medium were compared with SH medium. Protoplast yield from plants cultivated on SH medium was unambiguously better in case when donor plants were maintained and subcultured on the medium in the stage of intensive growth.The protoplast-derived calli were tested for response to the graded contents of the plant hormones with ratio 1:5 IAA:ZT and to the contents with different ratios from 1:4 to 1:25 IAA and ZT with aim to short time needed for organogenesis. The experiments were performed in tissue-culture plates with 4 x 6 wells. The application period was 90 days. The perspective combination was 0.1 mg/1 IAA, 2 mg/l ZT. The necessary time for start of shoot regeneration was in average 1 month shorter as compared to earlier experience. The positive effect was observed especially on protoplast-derived calli after electrofusion. A gradually increased concentration of growth regulators with the ratio 1:5 auxin:cytokinin did not result in plant regeneration."@en . "2"^^ . "Shortening of time of the plant regeneration from protoplast-derived calli in genus Solanum"@en . "2"^^ . . "RIV/60109807:_____/07:8P012140" . "1" . . . . . "RIV/60109807:_____/07:8P012140!RIV08-MSM-60109807" . "449719" . . "Polzerov\u00E1, Hana" . "CZ - \u010Cesk\u00E1 republika" . "61-69" . . "15" . "Shortening of time of the plant regeneration from protoplast-derived calli in genus Solanum" . . "Bylo prov\u00E1d\u011Bno srovn\u00E1n\u00ED kultivace rostlin na m\u00E9diu MS a na m\u00E9diu SH z hlediska v\u00FDt\u011B\u017Enosti izolovan\u00FDch protoplast\u016F. V\u00FDt\u011B\u017Enost protoplast\u016F izolovan\u00FDch z rostlin kultivovan\u00FDch na SH m\u00E9diu byla lep\u0161\u00ED v p\u0159\u00EDpad\u011B, \u017Ee kultivovan\u00E9 rostliny byly pas\u00E1\u017Eov\u00E1ny ve stadiu aktivn\u00EDho r\u016Fstu. U kalus\u016F protoplastov\u00E9ho p\u016Fvodu byla testov\u00E1na reakce na odstup\u0148ovan\u00E9 koncentrace 1:5 IAA:ZT a odli\u0161n\u00E9 pom\u011Bry 1:4 a\u017E 1:25 IAA a ZT za \u00FA\u010Delem zkr\u00E1cen\u00ED doby pot\u0159ebn\u00E9 k regeneraci rostlin na kalusech. Experimenty byly uskute\u010Dn\u011Bny v tk\u00E1\u0148ov\u00FDch desti\u010Dk\u00E1ch 4 x 6 jamek. R\u016Fstov\u00E9 regul\u00E1tory byly aplikov\u00E1ny v MS m\u00E9diu. Doba aplikace byla 90 dn\u016F. Perspektivn\u00ED kombinace byla 0,1 mg/1 IAA, 2 mg/l ZT. Doba pot\u0159ebn\u00E1 pro zah\u00E1jen\u00ED regenerace rostlin na kalusech se zkr\u00E1tila v pr\u016Fm\u011Bru o 1 m\u011Bs\u00EDc ve srovn\u00E1n\u00ED s p\u0159edchoz\u00EDmi zku\u0161enostmi. Pozitivn\u00ED efekt byl pozorov\u00E1n zejm\u00E9na na kalusech po f\u00FAz\u00EDch protoplast\u016F. Postupn\u011B zvy\u0161ovan\u00E1 koncentrace r\u016Fstov\u00FDch regul\u00E1tor\u016F v pom\u011Bru 1:5 auxin:cytokinin nevedla k regeneraci rostlin."@cs . "1802-940X" . "Zkr\u00E1cen\u00ED doby regenerace rostlin na kalusech protoplastov\u00E9ho p\u016Fvodu u rodu Solanum"@cs . . . . "Shortening of time of the plant regeneration from protoplast-derived calli in genus Solanum"@en . "Greplov\u00E1, Marie" . . . . . "Z(MSM6010980701)" . "[AB63DCDDEB1A]" .