"C\u00EDlem vlastn\u00ED pr\u00E1ce bylo vybrat a optimalizovat rychlou a citlivou laboratorn\u00ED metodu pro spolehlivou detekci tohoto karant\u00E9nn\u00EDho onemocn\u011Bn\u00ED bramboru. Jako experiment\u00E1ln\u00ED materi\u00E1ly byly vyu\u017Eity 2 typov\u00E9 izol\u00E1ty viroidn\u00ED v\u0159etenovitosti hl\u00EDz bramboru (PSTVd) ze sb\u00EDrky virov\u00FDch a viroidn\u00EDch patogen\u016F kat. \u010D. VIRUBRA 7/001 a 7/002 veden\u00E9 v in-vitro podm\u00EDnk\u00E1ch. Na\u0161e pr\u00E1ce porovn\u00E1vala metody izolace NA pomoc\u00ED magnetick\u00FDch \u010D\u00E1stic (MagExtractor-RNA Kit - Toyobo) s vyu\u017Eit\u00EDm p\u0159\u00EDstroje KingFisher mL a \u201Etradi\u010Dn\u00ED\u201C postup tj. izolace NA pomoc\u00ED komer\u010Dn\u00EDch souprav zalo\u017Een\u00FDch na specifick\u00E9 vazb\u011B a n\u00E1sledn\u00E9 eluci NA z nosi\u010D\u016F um\u00EDst\u011Bn\u00FDch ve speci\u00E1ln\u00EDch kolonk\u00E1ch (RNeasy Plant Total RNA Kit - Qiagen). RNA byla extrahov\u00E1na z rostlin in vitro. Ob\u011B zkou\u0161en\u00E9 metody poskytovaly RNA pro n\u00E1slednou RT-PCR bez zji\u0161t\u011Bn\u00ED vlivu p\u0159\u00EDtomnosti rostlinn\u00FDch inhibitor\u016F. P\u0159ednost\u00ED obou t\u011Bchto metod je jejich mal\u00E1 \u010Dasov\u00E1 n\u00E1ro\u010Dnost a srovnateln\u00E1 cena. Ob\u011B v\u00FD\u0161e zm\u00EDn\u011Bn\u00E9 techniky byly vhodn\u00E9 pro n\u00E1slednou RT-PCR."@cs . "Diagn\u00F3za viroidu v\u0159etenovitosti hl\u00EDz bramboru molekul\u00E1rn\u011B genetick\u00FDmi technikami (RT-PCR a RT-PCR v re\u00E1ln\u00E9m \u010Dase)" . "RIV/60109807:_____/06:#0000047" . "[A16D109646EF]" . "Diagn\u00F3za viroidu v\u0159etenovitosti hl\u00EDz bramboru molekul\u00E1rn\u011B genetick\u00FDmi technikami (RT-PCR a RT-PCR v re\u00E1ln\u00E9m \u010Dase)"@cs . "471448" . "Diagnosis of Potato spindle tuber viroid by means of molecular genetic techniques (RT-PCR and QRT-PCR)"@en . "\u010Cesk\u00E1 zem\u011Bd\u011Blsk\u00E1 univerzita v Praze" . "11"^^ . . . "Diagn\u00F3za viroidu v\u0159etenovitosti hl\u00EDz bramboru molekul\u00E1rn\u011B genetick\u00FDmi technikami (RT-PCR a RT-PCR v re\u00E1ln\u00E9m \u010Dase)"@cs . "37-39" . . "Praha" . "2006-01-01+01:00"^^ . "Praha" . "RIV/60109807:_____/06:#0000047!RIV07-MSM-60109807" . . . "1"^^ . "Z(MSM6010980701)" . . . . . "Diagn\u00F3za viroidu v\u0159etenovitosti hl\u00EDz bramboru molekul\u00E1rn\u011B genetick\u00FDmi technikami (RT-PCR a RT-PCR v re\u00E1ln\u00E9m \u010Dase)" . "Pt\u00E1\u010Dek, Ji\u0159\u00ED" . "The major aim of this work was to develope and evaluate rapid and sensitive method for the detection of PSTVd, because Potato spindle tuber viroid (PSTVd) is a quarantine pathogen in the European Union. According to the EU Plant Health directive 2000/29/EC, countries must be able to detect and identify this quarantine organism. Because viroids do not code for any protein, the RT-PCR or real-time RT-PCR assays are methods of choice suitable for large-scale testing. Also high throughput RNA isolation technique is needed for these tests. There were evaluated and adapted The EU protocol for this quarantine pathogen for the equipment in the laboratories of Potato Research Institute Havl\u00ED\u010Dk\u016Fv Brod, Czech Republic. We used evaluated RT-PCR and QRT-PCR procedures for detecting of PSTVd in tested samples. All the samples tested were negative."@en . . "Diagnosis of Potato spindle tuber viroid by means of molecular genetic techniques (RT-PCR and QRT-PCR)"@en . "Sborn\u00EDk abstrakt\u016F XVII. \u010Cesk\u00E9 a slovensk\u00E9 konference o ochran\u011B roslin" . . . "80-213-1516-4" . "C\u00EDlem vlastn\u00ED pr\u00E1ce bylo vybrat a optimalizovat rychlou a citlivou laboratorn\u00ED metodu pro spolehlivou detekci tohoto karant\u00E9nn\u00EDho onemocn\u011Bn\u00ED bramboru. Jako experiment\u00E1ln\u00ED materi\u00E1ly byly vyu\u017Eity 2 typov\u00E9 izol\u00E1ty viroidn\u00ED v\u0159etenovitosti hl\u00EDz bramboru (PSTVd) ze sb\u00EDrky virov\u00FDch a viroidn\u00EDch patogen\u016F kat. \u010D. VIRUBRA 7/001 a 7/002 veden\u00E9 v in-vitro podm\u00EDnk\u00E1ch. Na\u0161e pr\u00E1ce porovn\u00E1vala metody izolace NA pomoc\u00ED magnetick\u00FDch \u010D\u00E1stic (MagExtractor-RNA Kit - Toyobo) s vyu\u017Eit\u00EDm p\u0159\u00EDstroje KingFisher mL a \u201Etradi\u010Dn\u00ED\u201C postup tj. izolace NA pomoc\u00ED komer\u010Dn\u00EDch souprav zalo\u017Een\u00FDch na specifick\u00E9 vazb\u011B a n\u00E1sledn\u00E9 eluci NA z nosi\u010D\u016F um\u00EDst\u011Bn\u00FDch ve speci\u00E1ln\u00EDch kolonk\u00E1ch (RNeasy Plant Total RNA Kit - Qiagen). RNA byla extrahov\u00E1na z rostlin in vitro. Ob\u011B zkou\u0161en\u00E9 metody poskytovaly RNA pro n\u00E1slednou RT-PCR bez zji\u0161t\u011Bn\u00ED vlivu p\u0159\u00EDtomnosti rostlinn\u00FDch inhibitor\u016F. P\u0159ednost\u00ED obou t\u011Bchto metod je jejich mal\u00E1 \u010Dasov\u00E1 n\u00E1ro\u010Dnost a srovnateln\u00E1 cena. Ob\u011B v\u00FD\u0161e zm\u00EDn\u011Bn\u00E9 techniky byly vhodn\u00E9 pro n\u00E1slednou RT-PCR." . "PSTVd"@en . . "386"^^ . .