"Hylli\u0161, M." . . "Z(AV0Z60220518)" . "DNA 19 druh\u016F mikrosporidi\u00ED byla izolov\u00E1na a amplifikov\u00E1na z naka\u017Een\u00FDch hostitelsk\u00FDch tk\u00E1n\u00ED, kter\u00E9 byly p\u016Fvodn\u011B p\u0159ipraveny mezi lety 1946 - 1996. Trval\u00E9 prepar\u00E1ty na mikroskopick\u00FDch skl\u00ED\u010Dk\u00E1ch byly bu\u010F barveny Giemsou nebo nativn\u00ED. Giemsou barven\u00E9 prepar\u00E1ty, fixovan\u00E9 metanolem, se uk\u00E1zaly b\u00FDt vhodn\u00E9 k izolaci DNA. DNA byla \u00FAsp\u011B\u0161n\u011B izolov\u00E1na pouze ze dvou ze 14 nativn\u00EDch prepar\u00E1t\u016F. Izolovan\u00E1 DNA byla \u00FAsp\u011B\u0161n\u011B pou\u017Eita jako templ\u00E1t pro amplifikaci \u010D\u00E1st\u00ED mal\u00E9 a velk\u00E9 ribosom\u00E1ln\u00ED podjednotky rRNA, PCR amplikony pak byly n\u00E1sledn\u011B \u00FAsp\u011B\u0161n\u011B sekvenov\u00E1ny. Vysok\u00E1 \u00FA\u010Dinnost izolace DNA z archivn\u00EDch a sb\u00EDrkov\u00FDch prepar\u00E1t\u016F demonstruje vyu\u017Eitelnost t\u011Bchto sb\u00EDrek pro molekul\u00E1rn\u011B biologick\u00E9 a molekul\u00E1rn\u011B taxonomick\u00E9 studie."@cs . "RIV/60077409:_____/05:00024089" . "DNA from 19 species of microsporidia was isolated and amplified from infected host tissues that were originally prepared between the years 1946 and 1996. The smears, on glass microscope slides, were either Giemsa-stained or unstained. Methanol-fixed, Giemsa-stained smears proved to be suitable for DNA isolation; DNA was amplified from only two of 14 unstained slides. The isolated DNA was successfully amplified in PCRs using small subunit and large subunit rDNA primers and sequenced. The high efficiency of DNA isolation demonstrates the usefulness of archival and type collection slides for some molecular biology and molecular taxonomy studies."@en . "4"^^ . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "DNA isolation from museum and type collection slides of microsporidia"@en . "[226A53DB6BBA]" . . "Journal of Invertebrate Pathology" . "88" . "518478" . . "Oborn\u00EDk, Miroslav" . "2"^^ . . . . "DNA isolation from museum and type collection slides of microsporidia"@en . "microsporidia; Lepidoptera; phylogeny"@en . . "V\u00E1vra, Ji\u0159\u00ED" . . "Izolace DNA mikrosporidi\u00ED z trval\u00FDch mikroskopick\u00FDch prepar\u00E1t\u016F"@cs . . . "4"^^ . . "DNA isolation from museum and type collection slides of microsporidia" . "3" . . . "257;260" . . . "Izolace DNA mikrosporidi\u00ED z trval\u00FDch mikroskopick\u00FDch prepar\u00E1t\u016F"@cs . "RIV/60077409:_____/05:00024089!RIV06-AV0-60077409" . "DNA isolation from museum and type collection slides of microsporidia" . "Weiser, Jaroslav" . "DNA from 19 species of microsporidia was isolated and amplified from infected host tissues that were originally prepared between the years 1946 and 1996. The smears, on glass microscope slides, were either Giemsa-stained or unstained. Methanol-fixed, Giemsa-stained smears proved to be suitable for DNA isolation; DNA was amplified from only two of 14 unstained slides. The isolated DNA was successfully amplified in PCRs using small subunit and large subunit rDNA primers and sequenced. The high efficiency of DNA isolation demonstrates the usefulness of archival and type collection slides for some molecular biology and molecular taxonomy studies." . "0022-2011" .