. "1462-2912" . "0"^^ . . "ELF97 phosphate is a phosphatase substrate which produces ELFA, a fluorescent water-insoluble product, upon hydrolysis. We studied the kinetics of ELFA precipitation in acidic Ple\u0161n\u00E9 Lake (pH about 5; high phosphatase activity) and eutrophic \u0158\u00EDmov reservoir (pH 7-10; moderate phosphatase activity). Relative fluorescence ELFA precipitates was quantified with image analysis. Time-courses of ELFA formation exhibited lag periods followed by finite periods of linear increase. In Ple\u0161n\u00E9 Lake, lag-times were 1-18 min and rates of increase in ELFA fluorescence higher (by about 2 orders of magnitude) than in \u0158\u00EDmov reservoir (lag-times 30-200 min). Linear regression of seasonal data on rates of increase in ELFA fluorescence from image cytometry and spectrofluorometry (r (2) = 0.65, n = 10) allowed for calibration of image cytometry. Preliminary measurements of extracellular phosphatase activities of several algae resulted in rates 10-2260 fmol cell(-1) h(-1)."@en . "0"^^ . . . . "Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics."@en . . "5" . "Environmental Microbiology" . "6" . "606951" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Kom\u00E1rkov\u00E1, Jaroslava" . . "462;472" . . "RIV/60077387:_____/03:62033016" . . "[B788155F8988]" . . . . . "\u0160trojsov\u00E1, Alena" . "Nedoma, Ji\u0159\u00ED" . . "phosphorus; phytoplankton; lakes"@en . . . . "5"^^ . "\u0160imek, Karel" . "Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics." . . "ELF97 phosphate is a phosphatase substrate which produces ELFA, a fluorescent water-insoluble product, upon hydrolysis. We studied the kinetics of ELFA precipitation in acidic Ple\u0161n\u00E9 Lake (pH about 5; high phosphatase activity) and eutrophic \u0158\u00EDmov reservoir (pH 7-10; moderate phosphatase activity). Relative fluorescence ELFA precipitates was quantified with image analysis. Time-courses of ELFA formation exhibited lag periods followed by finite periods of linear increase. In Ple\u0161n\u00E9 Lake, lag-times were 1-18 min and rates of increase in ELFA fluorescence higher (by about 2 orders of magnitude) than in \u0158\u00EDmov reservoir (lag-times 30-200 min). Linear regression of seasonal data on rates of increase in ELFA fluorescence from image cytometry and spectrofluorometry (r (2) = 0.65, n = 10) allowed for calibration of image cytometry. Preliminary measurements of extracellular phosphatase activities of several algae resulted in rates 10-2260 fmol cell(-1) h(-1)." . . "11"^^ . "P(GA206/00/0063), P(IAA6017202), P(IBS6017004), Z(AV0Z6017912)" . "5"^^ . . "Vrba, Jaroslav" . . "RIV/60077387:_____/03:62033016!RIV/2004/AV0/A62004/N" . "Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics."@en . "Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics." . . .