. . . . "6"^^ . "2" . . "RIV/60077344:_____/14:00427515" . "6"^^ . . "756" . "Luke\u0161, Julius" . . "Arthropod ecology and taxonomy have witnessed a substantial increase in the use of various trapping and molecular methods in the past two decades. However, the methods of collecting and the preservation liquids most widely used in arthropod traps do not properly preserve DNA. A stable and cheap substance that can be used to trap arthropods in and preserve their DNA is therefore needed. We test whether SDS/EDTA water solution can preserve DNA of beetles for one, four and eight weeks. Preservation of DNA was tested using PCR amplification of parts of the mitochondrial cytochrome c oxidase I (Cox1) and nuclear 28S rRN A genes. All the solutions tested preserved DNA for at least up to eight weeks and we recommend 2% SDS and 100mM EDTA as a cheap, stable and easily transportable alternative to ethanol for preserving specimens and their DNA collected in the field. This solution is also suitable for using as the collection and preservation liquid in arthropod traps."@en . . "A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods" . . "Drag, Luk\u00E1\u0161" . . "000343664100003" . "[042B41C41B2F]" . . . . "CZ - \u010Cesk\u00E1 republika" . "A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods"@en . "111" . "10.14411/eje.2014.024" . "\u010C\u00ED\u017Eek, Luk\u00E1\u0161" . . . "Pokluda, Pavel" . . "A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods" . "St\u0159\u00EDbrn\u00E1, Eva" . . "Arthropod ecology and taxonomy have witnessed a substantial increase in the use of various trapping and molecular methods in the past two decades. However, the methods of collecting and the preservation liquids most widely used in arthropod traps do not properly preserve DNA. A stable and cheap substance that can be used to trap arthropods in and preserve their DNA is therefore needed. We test whether SDS/EDTA water solution can preserve DNA of beetles for one, four and eight weeks. Preservation of DNA was tested using PCR amplification of parts of the mitochondrial cytochrome c oxidase I (Cox1) and nuclear 28S rRN A genes. All the solutions tested preserved DNA for at least up to eight weeks and we recommend 2% SDS and 100mM EDTA as a cheap, stable and easily transportable alternative to ethanol for preserving specimens and their DNA collected in the field. This solution is also suitable for using as the collection and preservation liquid in arthropod traps." . "European Journal of Entomology" . "5"^^ . "I, P(GA204/09/1667), P(GA206/09/0115), P(GAP504/12/1952), P(TA02021501)" . . . "A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods"@en . "1210-5759" . . "Novotn\u00FD, Vojt\u011Bch" . "RIV/60077344:_____/14:00427515!RIV15-GA0-60077344" . . "insects; beetles; DNA preservation"@en . . . .