"US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "5"^^ . "Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato" . "Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato" . . . . "Kodr\u00EDk, Dalibor" . . "10.1007/s12010-013-0325-9" . "Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato"@en . . "Vinokurov, Konstantin" . "171" . . "000324109800018" . . "16"^^ . . "Kludkiewicz, Barbara" . "[41B7A2675E25]" . "Svobodov\u00E1, Z." . "100505" . "1" . . "Several recombinant derivatives of silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subtilisin and proteinase K. A construct of the gene was introduced into Agrobacterium tumefaciens that was then used for genetic transformation of the potato variety Velox. In vitro tests showed increased resistance to the late blight Phytophthora infestans in several transformed lines. No effect was seen on biological characteristics of Spodoptera littoralis indicating that the transformed potatoes may be harmless to non-target organisms." . . . "RIV/60077344:_____/13:00395286" . . "Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato"@en . . "Several recombinant derivatives of silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subtilisin and proteinase K. A construct of the gene was introduced into Agrobacterium tumefaciens that was then used for genetic transformation of the potato variety Velox. In vitro tests showed increased resistance to the late blight Phytophthora infestans in several transformed lines. No effect was seen on biological characteristics of Spodoptera littoralis indicating that the transformed potatoes may be harmless to non-target organisms."@en . . "Hor\u00E1\u010Dkov\u00E1, V." . . "Skokov\u00E1 Habu\u0161tov\u00E1, Oxana" . "0273-2289" . . . "Sehnal, Franti\u0161ek" . "Vinokurov, Konstantin" . "Applied Biochemistry and Biotechnology" . "8"^^ . "http://link.springer.com/content/pdf/10.1007%2Fs12010-013-0325-9.pdf" . . "Kludkiewicz, Barbara" . "Navr\u00E1til, Old\u0159ich" . . "genetically modified organism; silk protease inhibitor; protease"@en . "I, P(QI91A229), V" . "RIV/60077344:_____/13:00395286!RIV14-AV0-60077344" .