"PDMPO; bacterial colonization; diatoms; Fragilaria crotonensis; flood; reservoir"@en . . . "1" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . . "10.3354/ame01560" . "Bacterial colonization of the freshwater planktonic diatom Fragilaria crotonensis" . . "000302359100009" . "0948-3055" . . . "Bacterial colonization of the freshwater planktonic diatom Fragilaria crotonensis"@en . "Bacterial colonization of the freshwater planktonic diatom Fragilaria crotonensis" . "Aquatic Microbial Ecology" . "[BE111739E04D]" . "RIV/60077344:_____/12:00377857!RIV13-GA0-60077344" . "Nedoma, Ji\u0159\u00ED" . . "124378" . "3"^^ . . . . "3"^^ . "RIV/60077344:_____/12:00377857" . . . . "In 2006, the seasonal course of bacterial colonization and silification rates of a colonial diatom Fragilaria crotonensis were studied at two distinct depths (surface \u2013 0.5 m and Zeu \u2013 depth of 1% of surface PAR) in the eutrophic \u0158\u00EDmov Reservoir (Czech Republic). In the study period (July \u2013 September), Fragilaria dominated the phytoplankton and formed remarkable subsurface peaks of biomass at Zeu. Diatoms living under light limited conditions at Zeu displayed lower silification rates measured with the fluorescence probe 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) and were significantly more colonized by bacteria than at the surface (seasonal means 7.8 and 5.0 bacteria per Fragilaria cell, respectively). In three additional experiments using double stained Fragilaria cells (PDMPO + DAPI), diatom cells with no PDMPO fluorescence were significantly more colonized by bacteria regardless of the depth indicating that bacterial colonization reflects physiological state of the diatoms. As an apparent consequence of a flood event in August, Fragilaria assemblage was composed of two temporally isolated sub-populations differing markedly in the cell length, cell surface area and extent of bacterial colonization. Although both Fragilaria sub-populations showed similar silification rates, the late summer (post flood) Fragilaria sub-population with smaller cells showed a significantly lower bacterial colonization, while the difference in bacterial colonization between the depths remained consistently significant throughout the study." . . . . . "Znachor, Petr" . "Bacterial colonization of the freshwater planktonic diatom Fragilaria crotonensis"@en . "In 2006, the seasonal course of bacterial colonization and silification rates of a colonial diatom Fragilaria crotonensis were studied at two distinct depths (surface \u2013 0.5 m and Zeu \u2013 depth of 1% of surface PAR) in the eutrophic \u0158\u00EDmov Reservoir (Czech Republic). In the study period (July \u2013 September), Fragilaria dominated the phytoplankton and formed remarkable subsurface peaks of biomass at Zeu. Diatoms living under light limited conditions at Zeu displayed lower silification rates measured with the fluorescence probe 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) and were significantly more colonized by bacteria than at the surface (seasonal means 7.8 and 5.0 bacteria per Fragilaria cell, respectively). In three additional experiments using double stained Fragilaria cells (PDMPO + DAPI), diatom cells with no PDMPO fluorescence were significantly more colonized by bacteria regardless of the depth indicating that bacterial colonization reflects physiological state of the diatoms. As an apparent consequence of a flood event in August, Fragilaria assemblage was composed of two temporally isolated sub-populations differing markedly in the cell length, cell surface area and extent of bacterial colonization. Although both Fragilaria sub-populations showed similar silification rates, the late summer (post flood) Fragilaria sub-population with smaller cells showed a significantly lower bacterial colonization, while the difference in bacterial colonization between the depths remained consistently significant throughout the study."@en . . . "66" . . . "P(GA206/08/0015), P(GAP504/11/2177), P(GAP504/11/2182), Z(AV0Z60170517)" . . "\u0160imek, Karel" . . . "8"^^ .