. . "182905" . "621/4043" . "SVS/2012/013145-G" . "zv\u00FD\u0161en\u00ED ochrany" . "SVS/2012/013145-G" . . "4"^^ . . . . "RIV/60077344:_____/12:00377695" . "3"^^ . . "\u00DAst\u0159edn\u00ED veterin\u00E1rn\u00ED spr\u00E1va St\u00E1tn\u00ED veterin\u00E1rn\u00ED spr\u00E1vy Praha" . "This publication provides information on the methods of mosquito monitoring and detection of viruses. Capturing of mosquitoes \u2013 vectores of many diseases of mans and domestic animals depends on the type of trap and attractant used, another important factor is the height at which the trap is situated. Classical methods for arboviruses detection (suckling mouse brain inoculation, CPE in cell culture, etc.) are laborious and are biased towards viruses that grow well in vitro or in vivo. Thus, these classical methods are not always suitable for large-scale surveillance. PCR-based techniques have been shown to be efficient and sensitive in surveillance for individual virus species. We hope that this guide described methods of monitoring of mosquitoes and detection of viruses by RT-PCR will be helpful for the authority of protection public health. Use of these methods enable optimization of mosquitoes eradication by methods economically advantageous and saving to the nature."@en . . "Publikace d\u00E1v\u00E1 z\u00E1kladn\u00ED informace o metodice monitoringu v\u00FDskytu kom\u00E1r\u016F a o detekci vir\u016F. Odchyt homar\u016F \u2013 vektor\u016F mnoha onemocn\u011Bn\u00ED \u010Dlov\u011Bka i dom\u00E1c\u00EDch zv\u00ED\u0159at je z\u00E1visl\u00FD na typu pou\u017Eit\u00E9ho lapa\u010De a atraktantu. Dal\u0161\u00EDm d\u016Fle\u017Eit\u00FDm faktorem je v\u00FD\u0161ka zav\u011B\u0161en\u00ED lapa\u010De. Klasick\u00E9 metody pro detekci arbovir\u016F (inokulace na mozc\u00EDch my\u0161at, CPE bun\u011B\u010Dn\u00E9 kultu\u0159e, atd.) jsou pracn\u00E9 a z\u00E1visl\u00E9 na orientaci zda dob\u0159e rostou in vivo nebo in vitro. Proto nejsou klasick\u00E9 metody v\u017Edy vhodn\u00E9 pro rozs\u00E1hl\u00E1 pozorov\u00E1n\u00ED. PCR metodiky se uk\u00E1zaly jako efektivn\u00ED a senzitivn\u00ED p\u0159i sledov\u00E1n\u00ED jednotliv\u00FDch vir\u016F. Douf\u00E1me, \u017Ee tento pr\u016Fvodce popisuj\u00EDc\u00ED metody monitoringu kom\u00E1r\u016F a detekci vor\u016F pomoc\u00ED RT-PCr bude n\u00E1pomocen p\u0159i ochran\u011B zdrav\u00ED \u010Dlov\u011Bka. Pou\u017Eit\u00ED t\u011Bchto metod umo\u017En\u00ED optimalizaci eradikace kom\u00E1r\u016F ekonomicky v\u00FDhodn\u00FDmi a p\u0159\u00EDrodu chr\u00E1n\u00EDc\u00EDmi metodami."@cs . . . . "Gelbi\u010D, Ivan" . . . "Publikace d\u00E1v\u00E1 z\u00E1kladn\u00ED informace o metodice monitoringu v\u00FDskytu kom\u00E1r\u016F a o detekci vir\u016F. Odchyt homar\u016F \u2013 vektor\u016F mnoha onemocn\u011Bn\u00ED \u010Dlov\u011Bka i dom\u00E1c\u00EDch zv\u00ED\u0159at je z\u00E1visl\u00FD na typu pou\u017Eit\u00E9ho lapa\u010De a atraktantu. Dal\u0161\u00EDm d\u016Fle\u017Eit\u00FDm faktorem je v\u00FD\u0161ka zav\u011B\u0161en\u00ED lapa\u010De. Klasick\u00E9 metody pro detekci arbovir\u016F (inokulace na mozc\u00EDch my\u0161at, CPE bun\u011B\u010Dn\u00E9 kultu\u0159e, atd.) jsou pracn\u00E9 a z\u00E1visl\u00E9 na orientaci zda dob\u0159e rostou in vivo nebo in vitro. Proto nejsou klasick\u00E9 metody v\u017Edy vhodn\u00E9 pro rozs\u00E1hl\u00E1 pozorov\u00E1n\u00ED. PCR metodiky se uk\u00E1zaly jako efektivn\u00ED a senzitivn\u00ED p\u0159i sledov\u00E1n\u00ED jednotliv\u00FDch vir\u016F. Douf\u00E1me, \u017Ee tento pr\u016Fvodce popisuj\u00EDc\u00ED metody monitoringu kom\u00E1r\u016F a detekci vor\u016F pomoc\u00ED RT-PCr bude n\u00E1pomocen p\u0159i ochran\u011B zdrav\u00ED \u010Dlov\u011Bka. Pou\u017Eit\u00ED t\u011Bchto metod umo\u017En\u00ED optimalizaci eradikace kom\u00E1r\u016F ekonomicky v\u00FDhodn\u00FDmi a p\u0159\u00EDrodu chr\u00E1n\u00EDc\u00EDmi metodami." . "Accuracy improvement and standardization on the method of mosquito monitoring and procedure for detection of flaviviruses and bunyaviruses"@en . "Kilian, Patrik" . "RIV/60077344:_____/12:00377695!RIV13-GA0-60077344" . . "mosquito monitoring; arboviruses; detection of viruses"@en . . "[B5BF7ACB76C5]" . "\u0160ebesta, Old\u0159ich" . . "R\u016F\u017Eek, Daniel" . . "Zp\u0159esn\u011Bn\u00ED a standartizace metodiky monitoringu v\u00FDskytu kom\u00E1r\u016F a postupu pro detekci flavivir\u016F a bunyavir\u016F"@cs . . "2012-08-22+02:00"^^ . . "Accuracy improvement and standardization on the method of mosquito monitoring and procedure for detection of flaviviruses and bunyaviruses"@en . "Zp\u0159esn\u011Bn\u00ED a standartizace metodiky monitoringu v\u00FDskytu kom\u00E1r\u016F a postupu pro detekci flavivir\u016F a bunyavir\u016F"@cs . . "P(2B08003), P(GD206/09/H026), Z(AV0Z50070508), Z(AV0Z60220518), Z(AV0Z60930519)" . . . "Zp\u0159esn\u011Bn\u00ED a standartizace metodiky monitoringu v\u00FDskytu kom\u00E1r\u016F a postupu pro detekci flavivir\u016F a bunyavir\u016F" . . "Zp\u0159esn\u011Bn\u00ED a standartizace metodiky monitoringu v\u00FDskytu kom\u00E1r\u016F a postupu pro detekci flavivir\u016F a bunyavir\u016F" .