"RIV/60077344:_____/12:00377469" . . "123007" . "NL - Nizozemsko" . "1" . "\u0160pakov\u00E1, Vlastimila" . . . . "Plant Cell, Tissue and Organ Culture" . "\u0160pak, Josef" . . "Votruba, Ivan" . . . . . . . "Petrzik, Karel" . "4"^^ . "Hol\u00FD, Anton\u00EDn" . . "10" . . . . "P(GA522/09/0707), Z(AV0Z40550506), Z(AV0Z50510513)" . "000304664300007" . . "[3439D66F742D]" . . "Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis" . . "0167-6857" . . "Antiviral effects of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP, and ribavirin on double-stranded DNA Cauliflower mosaic virus (CaMV) were evaluated in Brassica pekinensis plants grown in vitro on liquid medium. A double-antibody sandwich ELISA was used for relative quantification of viral protein and PCR for detection of CaMV nucleic acid in plants. Ribavirin and PMEA had no significant antiviral effect. (S)-HPMPC at concentration 50 mg l -1 and PMEDAP at concentrations 50 and 12. 5 mg l -1 significantly (P < 0.05) reduced CaMV concentration in plants within 42-63 days to levels detectable neither by ELISA nor by PCR. A phytotoxicity experiment resulted in progressive yellowing of leaves and dwarfing in plants cultured 42 days on media with concentrations 12. 5, 25 and 50 mg l -1 of (S)-HPMPC and PMEDAP. Reduction in fresh and dry weights of plants was significant (P < 0. 05) already at 12. 5 mg l -1 with both compounds." . "RIV/60077344:_____/12:00377469!RIV13-GA0-60077344" . "Antiviral effects of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP, and ribavirin on double-stranded DNA Cauliflower mosaic virus (CaMV) were evaluated in Brassica pekinensis plants grown in vitro on liquid medium. A double-antibody sandwich ELISA was used for relative quantification of viral protein and PCR for detection of CaMV nucleic acid in plants. Ribavirin and PMEA had no significant antiviral effect. (S)-HPMPC at concentration 50 mg l -1 and PMEDAP at concentrations 50 and 12. 5 mg l -1 significantly (P < 0.05) reduced CaMV concentration in plants within 42-63 days to levels detectable neither by ELISA nor by PCR. A phytotoxicity experiment resulted in progressive yellowing of leaves and dwarfing in plants cultured 42 days on media with concentrations 12. 5, 25 and 50 mg l -1 of (S)-HPMPC and PMEDAP. Reduction in fresh and dry weights of plants was significant (P < 0. 05) already at 12. 5 mg l -1 with both compounds."@en . "10.1007/s11240-012-0130-0" . "6"^^ . . "Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis" . "Caulimovirus; Chemotherapy; Pararetrovirus; dsDNA"@en . . "6"^^ . . "Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis"@en . . "Pavingerov\u00E1, Daniela" . "Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis"@en .