"Golovchenko, Maryna" . "http://www.sciencedirect.com/science/article/pii/S0020751912000033" . . . . . "Rudenko, Natalia" . "42" . "Functional genomics studies of Rhipicephalus (Boophilus) annulatus ticks in response to infection with the cattle protozoan parasite, Babesia bigemina"@en . . . "Functional genomics studies of Rhipicephalus (Boophilus) annulatus ticks in response to infection with the cattle protozoan parasite, Babesia bigemina"@en . "RIV/60077344:_____/12:00377104" . . "10.1016/j.ijpara.2011.12.003" . "Tick; Genomics; Babesia; Rhipicephalus; Boophilus; RNA interference; Vaccine"@en . . "Functional genomics studies of Rhipicephalus (Boophilus) annulatus ticks in response to infection with the cattle protozoan parasite, Babesia bigemina" . "Z(AV0Z60220518)" . . "Functional genomics studies of Rhipicephalus (Boophilus) annulatus ticks in response to infection with the cattle protozoan parasite, Babesia bigemina" . . "9"^^ . "Domingos, A." . . "Almaz\u00E1n, C." . . . . "do Ros\u00E1rio, A." . . "3"^^ . "The objectives of this study were to identify Rhipicephalus annulatus genes differentially expressed in response to infection with Babesia bigemina. Functional analyses were conducted on selected genes by RNA interference in both R. annulatus and R. microplus ticks. 800 randomly selected suppression-subtractive hybridisation library clones were sequenced and analysed. Differentially expressed genes with putative functions in tick-pathogen interactions were selected for validation of SSH results by real-time reverse transcription-PCR. Genes encoding for TROSPA, calreticulin, ricinusin and serum amyloid A were over-expressed in B. bigemina-infected ticks while Kunitz-type protease inhibitor 5 mRNA levels were down-regulated in infected ticks. Functional analysis of differentially expressed genes by dsRNA-mediated RNAi showed that under the conditions of the present study knockdown of TROSPA and serum amyloid A significantly reduced B. bigemina infection levels in R. annulatus."@en . . . "The objectives of this study were to identify Rhipicephalus annulatus genes differentially expressed in response to infection with Babesia bigemina. Functional analyses were conducted on selected genes by RNA interference in both R. annulatus and R. microplus ticks. 800 randomly selected suppression-subtractive hybridisation library clones were sequenced and analysed. Differentially expressed genes with putative functions in tick-pathogen interactions were selected for validation of SSH results by real-time reverse transcription-PCR. Genes encoding for TROSPA, calreticulin, ricinusin and serum amyloid A were over-expressed in B. bigemina-infected ticks while Kunitz-type protease inhibitor 5 mRNA levels were down-regulated in infected ticks. Functional analysis of differentially expressed genes by dsRNA-mediated RNAi showed that under the conditions of the present study knockdown of TROSPA and serum amyloid A significantly reduced B. bigemina infection levels in R. annulatus." . "Antunes, S." . "Galindo, R. C." . "International Journal for Parasitology" . "AU - Australsk\u00E9 spole\u010Denstv\u00ED" . . . "10"^^ . "Grubhoffer, Libor" . "de la Fuente, J." . . . "Shkap, V." . "137379" . "RIV/60077344:_____/12:00377104!RIV13-AV0-60077344" . "000301820000007" . . "[7DE12DC54033]" . "0020-7519" . "2" .