"Ubiquity of Polynucleobacter necessarius subspecies asymbioticus results from ecological diversification" . . "ubiquity; Polynucleobacter; ecological diversification"@en . "RIV/60077344:_____/11:00358982!RIV12-MSM-60077344" . "RIV/60077344:_____/11:00358982" . . "236441" . "Hahn, M. W." . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "2"^^ . "Ubiquity of Polynucleobacter necessarius subspecies asymbioticus results from ecological diversification"@en . . "4"^^ . . . "Jezberov\u00E1, Jitka" . . "Brandt, U." . "Ubiquity of Polynucleobacter necessarius subspecies asymbioticus results from ecological diversification" . "The subspecies Polynucleobacter necessarius asymbioticus (> 99% 16S rRNA similarity) has a cosmopolitan distribution and a ubiquitous occurrence in freshwater habitats. We tested if the observed ubiquity of these free-living planktonic freshwater bacteria results from a euryoecious (generalist) adaptation of P. n. asymbioticus strains, or from ecological diversification within the subspecies. We developed a reverse line blot hybridization assay enabling the cultivation-independent detection of 13 groups within the subspecies in environmental samples. A set of 121 lentic freshwater habitats, spanning a broad variety of habitat types (e.g. pH levels ranging from 3.8 to 8.5) was investigated for the presence of these 13 P. n. asymbioticus groups. Statistical analyses of the RLBH detections revealed pronounced differences in habitat preferences of several of the groups. Their preferences differed regarding pH, conductivity, dissolved organic carbon and oxygen concentration of habitats."@en . "4" . . . . "Jezbera, Jan" . "1462-2912" . "The subspecies Polynucleobacter necessarius asymbioticus (> 99% 16S rRNA similarity) has a cosmopolitan distribution and a ubiquitous occurrence in freshwater habitats. We tested if the observed ubiquity of these free-living planktonic freshwater bacteria results from a euryoecious (generalist) adaptation of P. n. asymbioticus strains, or from ecological diversification within the subspecies. We developed a reverse line blot hybridization assay enabling the cultivation-independent detection of 13 groups within the subspecies in environmental samples. A set of 121 lentic freshwater habitats, spanning a broad variety of habitat types (e.g. pH levels ranging from 3.8 to 8.5) was investigated for the presence of these 13 P. n. asymbioticus groups. Statistical analyses of the RLBH detections revealed pronounced differences in habitat preferences of several of the groups. Their preferences differed regarding pH, conductivity, dissolved organic carbon and oxygen concentration of habitats." . . . "[729157C7C63D]" . . "Environmental Microbiology" . "000289116700008" . . . "10.1111/j.1462-2920.2010.02396.x" . "13" . . . "10"^^ . . "Ubiquity of Polynucleobacter necessarius subspecies asymbioticus results from ecological diversification"@en . "P(GAP504/10/0566), P(MEB060901), Z(AV0Z60170517)" .