. . . "\u0160mahel, M." . "Enhancement of T cell-mediated and humoral immunity of beta-glucuronidase-based DNA vaccines against HPV16 E7 oncoprotein" . . "In this study, we attempted to improve immunogenicity of the GUS-based anti-E7 vaccines by increasing the steady-state level of fusion proteins. We fused deletion mutants of E7GGG and codon-optimized E7GGG with the 5'-terminus of GUS and unaltered E7GGG with the 3'-terminus of GUS. Furthermore, we mutated the initiation codon of the GUS gene in the E7GGG.GUS construct, as GUS alone was produced from this fusion gene." . "GR - \u0158eck\u00E1 republika" . "1"^^ . . . "1019-6439" . "Enhancement of T cell-mediated and humoral immunity of beta-glucuronidase-based DNA vaccines against HPV16 E7 oncoprotein"@en . "International Journal of Oncology" . "000257276200010" . "Vlas\u00E1k, Josef" . . "In this study, we attempted to improve immunogenicity of the GUS-based anti-E7 vaccines by increasing the steady-state level of fusion proteins. We fused deletion mutants of E7GGG and codon-optimized E7GGG with the 5'-terminus of GUS and unaltered E7GGG with the 3'-terminus of GUS. Furthermore, we mutated the initiation codon of the GUS gene in the E7GGG.GUS construct, as GUS alone was produced from this fusion gene."@en . "RIV/60077344:_____/08:00321729" . "Enhancement of T cell-mediated and humoral immunity of beta-glucuronidase-based DNA vaccines against HPV16 E7 oncoprotein" . "10"^^ . "[A2632AFE6146]" . "Ludv\u00EDkov\u00E1, V." . "366264" . . "V t\u00E9to pr\u00E1ci jsme se pokusili zlep\u0161it imunogenitu na GUS zalo\u017Een\u00E9 anti-E7 vakc\u00EDny zv\u00FD\u0161en\u00EDm rovnov\u00E1\u017En\u00E9 koncentrace tohoto f\u00FAzn\u00EDho proteinu. S 5' koncem GUS jsme fuzovali dele\u010Dn\u00ED mutanty E7GGG a kodonov\u011B optimalizovan\u00FD E7GGG, s 5' koncem GUS pak nezm\u011Bn\u011Bn\u00FD E7GGG. D\u00E1le jsme mutovali inicia\u010Dn\u00ED kodon genu GUS v konstruktu E7GGG.GUS, nebo\u0165 produktem tohoto f\u00FAzn\u00EDho genu byl samotn\u00FD protein GUS."@cs . . . "33" . . "Zv\u00FD\u0161en\u00ED T bu\u0148kami zprost\u0159edkovan\u00E9 a humor\u00E1ln\u00ED imunity proti onkoproteinu E7 z HPV DNA vakc\u00EDnou zalo\u017Eenou na beta-glucuronid\u00E1ze"@cs . "Zv\u00FD\u0161en\u00ED T bu\u0148kami zprost\u0159edkovan\u00E9 a humor\u00E1ln\u00ED imunity proti onkoproteinu E7 z HPV DNA vakc\u00EDnou zalo\u017Eenou na beta-glucuronid\u00E1ze"@cs . . . "RIV/60077344:_____/08:00321729!RIV09-GA0-60077344" . "1" . . "Enhancement of T cell-mediated and humoral immunity of beta-glucuronidase-based DNA vaccines against HPV16 E7 oncoprotein"@en . . . . "Pol\u00E1kov\u00E1, I." . "immunogenicity; anti-E7 vaccines"@en . "Pokorn\u00E1, D." . "P(GA521/05/2092), P(GD310/05/H533), P(NR9246), Z(AV0Z50510513)" . "6"^^ . "Du\u0161kov\u00E1, M." . .