"[1FB021582298]" . . "3" . . . "52" . . "The fluorogenic substrate Enzyme-Labeled Fluorescence 97 (ELF-P) is hydrolyzed by the P-cleaving enzyme phosphatase, producing ELF 97 alcohol (ELFA), a fluorescent-insoluble product. A high percentage of the cultured cells were labeled with ELFA (the ratio of ELFA spots to total DAPI counts in P-limited cultures ranged from 26 to 100%, depending on the strain). In contrast, this ratio never exceeded 0.01% in Mediterranean samples, even when P was demonstrated to be a significant limiting factor." . "RIV/60077344:_____/08:00317620!RIV09-AV0-60077344" . "7"^^ . "Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea" . . "RIV/60077344:_____/08:00317620" . "Aktivita alkalick\u00FDch fosfat\u00E1z mo\u0159sk\u00FDch bakteri\u00ED studovan\u00E1 pomoc\u00ED substr\u00E1tu ELF97: \u00FAsp\u011Bchy a meze pou\u017Eitelnosti metody ve fosforem limitovan\u00E9m St\u0159edozemn\u00EDm mo\u0159i"@cs . "Van Wambeke, F." . "Aktivita alkalick\u00FDch fosfat\u00E1z mo\u0159sk\u00FDch bakteri\u00ED studovan\u00E1 pomoc\u00ED substr\u00E1tu ELF97: \u00FAsp\u011Bchy a meze pou\u017Eitelnosti metody ve fosforem limitovan\u00E9m St\u0159edozemn\u00EDm mo\u0159i"@cs . "The fluorogenic substrate Enzyme-Labeled Fluorescence 97 (ELF-P) is hydrolyzed by the P-cleaving enzyme phosphatase, producing ELF 97 alcohol (ELFA), a fluorescent-insoluble product. A high percentage of the cultured cells were labeled with ELFA (the ratio of ELFA spots to total DAPI counts in P-limited cultures ranged from 26 to 100%, depending on the strain). In contrast, this ratio never exceeded 0.01% in Mediterranean samples, even when P was demonstrated to be a significant limiting factor."@en . "000259980500004" . . "Fluorogenn\u00ED substr\u00E1t ELF97 fosf\u00E1t je hydrolyzov\u00E1n enzymem fosfat\u00E1zou p\u0159i\u010Dem\u017E vznik\u00E1 fluorescen\u010Dn\u00ED nerozpustn\u00FD produkt ELF 97 alkohol (ELFA). Ve fosforem limitovan\u00FDch bakteri\u00E1ln\u00EDch kultur\u00E1ch bylo vysok\u00E9 procento bun\u011Bk zna\u010Deno ELFA (26\u2013100% v z\u00E1vislosti na kmenu). Naproti tomu ve vzorc\u00EDch ze St\u0159edozemn\u00EDho mo\u0159e to bylo jen 0,01%, p\u0159esto \u017Ee byla prok\u00E1z\u00E1na limitace fosforem."@cs . "4"^^ . "Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea"@en . . . "Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea"@en . "Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea" . . "Aquatic Microbial Ecology" . "Nedoma, Ji\u0159\u00ED" . "Duhamel, S." . "1"^^ . . . . "Lebaron, P." . . . . "Z(AV0Z60170517)" . "marine bacteria; alkaline phosphatase; ELF97 phosphatase substrate"@en . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "355428" . "0948-3055" .