. "454;460" . . "\u0160trojsov\u00E1, Alena" . "1323-1650" . "Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments" . . "[5D99F62681C2]" . . . . "\u0160trojsov\u00E1, Martina" . "7"^^ . "ELF97 phosphate; microbial phosphatases; affinity"@en . . . "RIV/60077344:_____/07:00086809" . "P(IAA600170602), P(IAA6017202), V, Z(AV0Z60170517)" . "Studovali jsme afinitu extracelul\u00E1rn\u00EDch fosfat\u00E1z pro substr\u00E1t ELF97 fosf\u00E1t (ELFP) v r\u016Fzn\u00FDch akvatick\u00FDch ekosyst\u00E9mech nep\u0159\u00EDmou metodou zalo\u017Eenou na inhibici hydrol\u00FDzy 4-metylumbeliferyl fosf\u00E1tu. Hdnoty inhibi\u010Dn\u00ED konstanty, Ki, kter\u00E9 odpov\u00EDdaj\u00ED koncentraci pot\u0159ebn\u00E9 pro 50-% saturaci enzymu ELFP, byly nejni\u017E\u0161\u00ED v oligotrofn\u00ED oblasti St\u0159edozemn\u00EDho mo\u0159e, vy\u0161\u0161\u00ED v acidifikovan\u00FDch jezerech, eutrofn\u00ED p\u0159ehrad\u011B a v kultu\u0159e bakterie Alteromonas infernus. Na\u0161e pokusy ukazuj\u00ED \u017Ee koncetrace ELFP v kitu na m\u011B\u0159en\u00ED endogenn\u00EDch fosfat\u00E1z (Molecular Probes) zaji\u0161\u0165uje >99% saturaci extracelul\u00E1rn\u00EDch fosfat\u00E1z v mo\u0159i a >90% saturaci ve sladk\u00FDch vod\u00E1ch."@cs . "58" . "Van Wambeke, F." . "RIV/60077344:_____/07:00086809!RIV08-AV0-60077344" . "Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments"@en . . . . "5" . "Marine and Freshwater Research" . . "5"^^ . . . . "AU - Australsk\u00E9 spole\u010Denstv\u00ED" . "Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments" . "We indirectly studied the affinity of extracellular phosphatases for ELF97 phosphate (ELFP) in different aquatic environments through its ability to inhibit the hydrolysis of 4-methylumbelliferyl phosphate. Values of inhibition constants, Ki, which correspond to the concentrations necessary for half saturation of phosphatases by ELFP,were lowest in the oligotrophic Mediterranean Sea.We found higher values in oligo- to mesotrophic acidified lakes, in a eutrophic reservoir and in a pure culture of the marine bacterium Alteromonas infernus. Our experiments indicated that the assay ELFP concentration in the endogeneous phosphatase detection kit (Molecular Probes) used would ensure >99% saturation of extracellular phosphatases in marine environments and >90% saturation in the studied fresh waters."@en . "Duhamel, S." . "408715" . "Nedoma, Ji\u0159\u00ED" . "Afinita extracelul\u00E1rn\u00EDch fosfat\u00E1z pro ELF97 fosf\u00E1t ve vodn\u00EDm prost\u0159ed\u00ED"@cs . . "Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments"@en . "3"^^ . . . "We indirectly studied the affinity of extracellular phosphatases for ELF97 phosphate (ELFP) in different aquatic environments through its ability to inhibit the hydrolysis of 4-methylumbelliferyl phosphate. Values of inhibition constants, Ki, which correspond to the concentrations necessary for half saturation of phosphatases by ELFP,were lowest in the oligotrophic Mediterranean Sea.We found higher values in oligo- to mesotrophic acidified lakes, in a eutrophic reservoir and in a pure culture of the marine bacterium Alteromonas infernus. Our experiments indicated that the assay ELFP concentration in the endogeneous phosphatase detection kit (Molecular Probes) used would ensure >99% saturation of extracellular phosphatases in marine environments and >90% saturation in the studied fresh waters." . "Afinita extracelul\u00E1rn\u00EDch fosfat\u00E1z pro ELF97 fosf\u00E1t ve vodn\u00EDm prost\u0159ed\u00ED"@cs . . . .