"Fylogenetick\u00E1 slo\u017Een\u00ED a pod\u00EDl bakteri\u00ED p\u0159ij\u00EDmaj\u00EDc\u00EDch leucin byly studov\u00E1ny pomoc\u00ED metody fluorescen\u010Dn\u00ED in situ hybridizace kombinovan\u00E9 s mikroautoradiografi\u00ED. Bakterie byly ve sladkovodn\u00ED p\u0159ehradn\u00ED n\u00E1dr\u017Ei vystaveny r\u016Fzn\u00E9mu stupni dostupnosti \u017Eivin a predace prvok\u016F. Samotn\u00E9 bakterie nebo bakterie spolu s prvoky byly inkubov\u00E1ny v \u010D\u00E1stech n\u00E1dr\u017Ee limitovan\u00E9 a bohat\u00E9 na fosfor v dialyza\u010Dn\u00EDch s\u00E1\u010Dc\u00EDch, v lahv\u00EDch bez a s p\u0159\u00EDdavkem fosforu. Betaproteobacteria, zejm\u00E9na podskupina R-BT065, byly hlavn\u00EDmi skupinami bakteri\u00ED p\u0159ij\u00EDmaj\u00EDc\u00ED leucin ve v\u0161ech variant\u00E1ch a to p\u0159edev\u0161\u00EDm za podn\u00EDnek zv\u00FD\u0161en\u00E9 predace prvok\u016F a vysok\u00E9 dostupnosti \u017Eivin. Pouze velmi m\u00E1lo bun\u011Bk ze skupiny Sphingobacteria/Flavobacteria p\u0159ij\u00EDmalo leucin. A\u010Dkoli byla Gammaproteobacteria nejm\u00E9n\u011B po\u010Detnou skupinou, pat\u0159ila mezi vysoce aktivn\u00ED \u010D\u00E1st spole\u010Denstva bakteri\u00ED."@cs . "leucine incorporation; bacterial structure; bacterial function"@en . . "Vliv dostupnosti \u017Eivin a predace na p\u0159\u00EDjem leucinu r\u016Fzn\u00FDch skupin sladkovodn\u00EDch bakteri\u00ED studovan\u00E9 pomoc\u00ED mikroautoradiografie"@cs . "473364" . "4"^^ . . "3" . . "\u0160imek, Karel" . . "Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography" . . "Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography" . "Fluorescence in situ hybridization combined with microautoradiography was used to determine bacterial phylogenetic affiliations and the proportions of cells incorporating leucine. Bacteria were exposed to variable levels of available resources and bacterivory in a freshwater reservoir. Grazing-free and grazing-enhanced treatments were incubated in both P-limited and P-rich areas of the reservoir using dialysis bags, and bottle treatments with and without phosphorus enrichment. Betaproteobacteria, mainly the R-BT065 subcluster, become the dominant leucine-active bacteria in all treatments, in particular under conditions of enhanced protistan grazing and resource availability. The Sphingobacteria/Flavobacteria group exhibited very low rates of leucine uptake. Gammaproteobacteria was the least abundant but a highly active part of bacterial assemblage." . "RIV/60077344:_____/06:00076384" . "Hor\u0148\u00E1k, Karel" . . . "Fluorescence in situ hybridization combined with microautoradiography was used to determine bacterial phylogenetic affiliations and the proportions of cells incorporating leucine. Bacteria were exposed to variable levels of available resources and bacterivory in a freshwater reservoir. Grazing-free and grazing-enhanced treatments were incubated in both P-limited and P-rich areas of the reservoir using dialysis bags, and bottle treatments with and without phosphorus enrichment. Betaproteobacteria, mainly the R-BT065 subcluster, become the dominant leucine-active bacteria in all treatments, in particular under conditions of enhanced protistan grazing and resource availability. The Sphingobacteria/Flavobacteria group exhibited very low rates of leucine uptake. Gammaproteobacteria was the least abundant but a highly active part of bacterial assemblage."@en . "[098CEC68899F]" . . . . "Aquatic Microbial Ecology" . . . "Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography"@en . "277;289" . . . "Gasol, J. M." . . "13"^^ . "0948-3055" . . "Jezbera, Jan" . "5"^^ . "Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography"@en . . "P(GA206/05/0007), V, Z(AV0Z60170517)" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Nedoma, Ji\u0159\u00ED" . . "RIV/60077344:_____/06:00076384!RIV07-AV0-60077344" . . . "45" . "Vliv dostupnosti \u017Eivin a predace na p\u0159\u00EDjem leucinu r\u016Fzn\u00FDch skupin sladkovodn\u00EDch bakteri\u00ED studovan\u00E9 pomoc\u00ED mikroautoradiografie"@cs . .