. "\u0160imek, Petr" . "162" . "IE - Irsko" . "P(GA303/00/0923), Z(AV0Z50070508)" . "Toxicology Letters" . . "N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans" . . "0378-4274" . "6"^^ . . . . "Metabolism of the solvents N,N-dimethylformamide (DMF) and N-methylformamide (MF) results in the formation of N-methylcarbamoyl adducts at the N-terminal valine and lysine in blood protein globin. Globin from exposed subjects to DMF (n = 35) and unexposed controls (n = 5) was analyzed by 2 methods. Edman degradation was used as to measure the valine adduct by converting it to 3-methyl-5-isopropylhydantoin (MVH). The MVH levels in globin of the exposed subjects were in the range of 1-441 nmol/g, in controls < 1 nmol/g. The principal method involved enzymatic hydrolysis with pronase to release free N-epsilon-(N-methylcarbamoyl)lysine (MLU) and N-methyl-carbamoylvaline (MVU), which were directly determined by HPLC/MS/MS. MLU was found in most globins from the exposed subjects but not in the controls. A close correlation between the MLU and MVH levels (nmol/g) was observed: MLU = 7 + 0.48 MVH (R-2 = 0.84, n = 32)." . . "8"^^ . "Metabolismus rozpou\u0161t\u011Bdel N,N-dimethylformamidu (DMF) a N-methylformamidu (MF) vede ke vzniku N-methylkarbamoylov\u00FDch adukt\u016F s krevn\u00EDm globinem na N-termin\u00E1lov\u00E9m valinu a lysinu. Globin osob vystaven\u00FDch DMF (n = 35 ) a kontroly (n = 5) byly analyzov\u00E1ny dv\u011Bma metodami. Edmanova degradace byla pou\u017Eita k m\u011B\u0159en\u00ED valinov\u00E9ho aduktu 3-methyl-5-isopropylhydantoinu (MVH). MVH hladiny u exponovan\u00FDch osob byly v rozmez\u00ED 1-441 nmol/g, v kontrol\u00E1ch < 1 nmol/g. Hlavn\u00ED pou\u017Eit\u00E1 metoda zahrnovala enzymatickou pron\u00E1zovou hydrol\u00FDzu a n\u00E1slednou HPLC/M/MS anal\u00FDzu N-epsilon-(N-methylkarbamoyl)lysinu (MLU) and N-methyl-karbamoylvaline (MVU). MLU byl nalezen ve v\u011Bt\u0161in\u011B globin\u016F u exponovan\u00FDch subjekt\u016F, ale ne v kontrol\u00E1ch. Byla pozorov\u00E1na bl\u00EDzk\u00E1 korelace mezi hladinami MLU a MVH (nmol/g) : MLU = 7 + 0.48 MVH (R-2 = 0.84, n = 32). Poznatky nazna\u010Duj\u00ED dlouhodobou persitenci MLU v lidsk\u00E9m t\u011Ble, a tak jeho potenci\u00E1l poslou\u017Eit jako biomarker chronick\u00E9 expozice DMF."@cs . "Metabolism of the solvents N,N-dimethylformamide (DMF) and N-methylformamide (MF) results in the formation of N-methylcarbamoyl adducts at the N-terminal valine and lysine in blood protein globin. Globin from exposed subjects to DMF (n = 35) and unexposed controls (n = 5) was analyzed by 2 methods. Edman degradation was used as to measure the valine adduct by converting it to 3-methyl-5-isopropylhydantoin (MVH). The MVH levels in globin of the exposed subjects were in the range of 1-441 nmol/g, in controls < 1 nmol/g. The principal method involved enzymatic hydrolysis with pronase to release free N-epsilon-(N-methylcarbamoyl)lysine (MLU) and N-methyl-carbamoylvaline (MVU), which were directly determined by HPLC/MS/MS. MLU was found in most globins from the exposed subjects but not in the controls. A close correlation between the MLU and MVH levels (nmol/g) was observed: MLU = 7 + 0.48 MVH (R-2 = 0.84, n = 32)."@en . "Ki\u010Dov\u00E1, R." . "Nohov\u00E1, H." . "N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans"@en . "2"^^ . . "RIV/60077344:_____/06:00047087" . . "Cimlov\u00E1, Jana" . . . "N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans" . "N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans"@en . "2-3" . "Str\u00E1nsk\u00FD, V." . "RIV/60077344:_____/06:00047087!RIV07-AV0-60077344" . . . . "211;218" . "Adukt N-Methylkarbamoyl-lysinu v hemoglobinu: nov\u00FD metabolick\u00FD produkt a potenci\u00E1ln\u00ED biomarker expozice N,N-dimethylformamidu u \u010Dlov\u011Bka"@cs . . "Adukt N-Methylkarbamoyl-lysinu v hemoglobinu: nov\u00FD metabolick\u00FD produkt a potenci\u00E1ln\u00ED biomarker expozice N,N-dimethylformamidu u \u010Dlov\u011Bka"@cs . . . "[D0AC7AA516A4]" . . "Mr\u00E1z, J." . "488732" . . "N,N-dimethylformamide; globin; adducts"@en .