"Activity of extracellular acid phosphatases was measured at single-cell level in bacterioplankton groups defined by their morphology and size, in acidified mountain Lake \u010Certovo, using the substrate ELF97 phosphate. Fluorescent precipitates resulting from the substrate hydrolysis by phosphatases were quantified by image analysis. A conspicuous morphotype of curved cells, despite its low total biomass (average of 4%), contributed significantly (in average by 31%) to the total bacterioplankton phosphatase activity. Bacterial filaments, although comprising in average 85% of bacterioplankton biomass, contributed to the total bacterioplankton activity only by 45%. Biomass-specific activity of extracellular (cell-surface) phosphatases of the main bacterioplankton morphotypes increased in the order: filaments < cocci and rods < curved cells. The biomass-specific activity of bacterioplankton extracellular phosphatases was generally highest in the spring and decreased gradually during summer." . . "1271;1279" . "Specifick\u00E1 aktivita fosfat\u00E1zy v\u00E1zan\u00E9 na povrch bun\u011Bk u r\u016Fzn\u00FDch morfotyp\u016F bakterioplanktonu v acidifikovan\u00E9m horsk\u00E9m jeze\u0159e"@cs . "Environmental Microbiology" . "RIV/60077344:_____/06:00042946!RIV07-AV0-60077344" . "Nedoma, Ji\u0159\u00ED" . "Vrba, Jaroslav" . "Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake" . . "Activity of extracellular acid phosphatases was measured at single-cell level in bacterioplankton groups defined by their morphology and size, in acidified mountain Lake \u010Certovo, using the substrate ELF97 phosphate. Fluorescent precipitates resulting from the substrate hydrolysis by phosphatases were quantified by image analysis. A conspicuous morphotype of curved cells, despite its low total biomass (average of 4%), contributed significantly (in average by 31%) to the total bacterioplankton phosphatase activity. Bacterial filaments, although comprising in average 85% of bacterioplankton biomass, contributed to the total bacterioplankton activity only by 45%. Biomass-specific activity of extracellular (cell-surface) phosphatases of the main bacterioplankton morphotypes increased in the order: filaments < cocci and rods < curved cells. The biomass-specific activity of bacterioplankton extracellular phosphatases was generally highest in the spring and decreased gradually during summer."@en . "RIV/60077344:_____/06:00042946" . . "Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake" . "8" . "[12E9D4ADC007]" . "Specifick\u00E1 aktivita fosfat\u00E1zy v\u00E1zan\u00E9 na povrch bun\u011Bk u r\u016Fzn\u00FDch morfotyp\u016F bakterioplanktonu v acidifikovan\u00E9m horsk\u00E9m jeze\u0159e"@cs . "9"^^ . "2"^^ . . . . "2"^^ . "1462-2912" . "500687" . . . "alkaline phosphatase; bacterial morphorypes; acidified lake"@en . . "7" . . . . "V acidifikovan\u00E9m \u010Certov\u011B jeze\u0159e byla pomoc\u00ED fluorogenn\u00EDho substr\u00E1tu ELF97m\u011B\u0159ena za pou\u017Eit\u00ED pomoc\u00ED anal\u00FDzy obrazu aktivita kysel\u00FDch fosfat\u00E1z v\u00E1zan\u00FDch na povrch bakteri\u00E1ln\u00EDch bun\u011Bk. Najvy\u0161\u0161\u00ED aktivita byla zji\u0161t\u011Bna u specifick\u00E9ho morfotypu zahnut\u00FDch bun\u011Bk, jejich\u017E pod\u00EDl na biomase bakterioplanktonu \u010Dinil v sezonn\u00EDm pr\u016Fm\u011Bru 4%, zat\u00EDmco jejich pod\u00EDl na aktivit\u011B enzymu byl 31%. Vl\u00E1knit\u00E9 bakterie tvo\u0159ily naopak 85% biomasy, ale jejich pod\u00EDl na aktivit\u011B enzymu byl jen 45%. Specifick\u00E1 aktivita vzta\u017Een\u00E1 na biomasu byla nejvy\u0161\u0161\u00ED na ja\u0159e a sni\u017Eovala se v pr\u016Fb\u011Bhu sezony, byla nejvy\u0161\u0161\u00ED u zahnut\u00FDch bun\u011Bk, st\u0159edn\u00ED u kok\u00E1ln\u00EDch a ty\u010Dinkovit\u00FDch bun\u011Bk, a nejni\u017E\u0161\u00ED u vl\u00E1ken."@cs . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . . . . . "P(IAA6017202), V, Z(AV0Z60170517)" . "Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake"@en . . . "Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake"@en .