"RIV/60076658:12640/09:00010037!RIV10-MSM-12640___" . "V" . "Pascher, Torbjorn" . "Yartsev, Arkady" . "RIV/60076658:12640/09:00010037" . . . "Flash Photolysis of Cutinase: Identification and Decay Kinetics of Transient Intermediates Formed upon UV Excitation of Aromatic Residues"@en . "16"^^ . "[1CE5E2A353F1]" . "Flash Photolysis of Cutinase: Identification and Decay Kinetics of Transient Intermediates Formed upon UV Excitation of Aromatic Residues" . "97" . . "12640" . . "Biophysical Journal" . "Pol\u00EDvka, Tom\u00E1\u0161" . "8"^^ . . . . "EXCITED-STATE CHEMISTRY; ABSORBING AMINO-ACIDS; HYDRATED ELECTRON; AQUEOUS-SOLUTIONS; PULSE-RADIOLYSIS; LIQUID WATER; SIMPLE DERIVATIVES; GLOBAL ANALYSIS; RATE CONSTANTS; TRYPTOPHAN"@en . . . "1" . "1"^^ . "Skovsen, Esben" . . . . "Aromatic amino acids play an important role in ultraviolet (UV)-induced photochemical reactions in proteins. In this work, we aim at gaining insight into the photochemical reactions induced by near-UV light excitation of aromatic residues that lead to breakage of disulfide bridges in our model enzyme, Fusarium solani pisi cutinase, a lipolytic enzyme. With this purpose, we acquired transient absorption data of cutinase, with supplemental experimental data on tryptophan (Trp) and lysozyme as reference molecules. We here report formation kinetics and lifetimes of transient chemical species created upon UV excitation of aromatic residues in proteins. Two proteins, lysozyme and cutinase, as well as the free amino acid Trp, were studied under acidic, neutral, and alkaline conditions. The shortest-lived species is assigned to solvated electrons (lifetimes of a few microseconds to nanoseconds), whereas the longer-lived species are assigned to aromatic neutral and ionic radicals, Trp triplet states, and radic" . "Flash Photolysis of Cutinase: Identification and Decay Kinetics of Transient Intermediates Formed upon UV Excitation of Aromatic Residues"@en . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Sundstrom, Villly" . "Flash Photolysis of Cutinase: Identification and Decay Kinetics of Transient Intermediates Formed upon UV Excitation of Aromatic Residues" . "Klitgaard, Soren" . . . "Petersen, Steffen B" . "000267871000021" . "0006-3495" . . . "Aromatic amino acids play an important role in ultraviolet (UV)-induced photochemical reactions in proteins. In this work, we aim at gaining insight into the photochemical reactions induced by near-UV light excitation of aromatic residues that lead to breakage of disulfide bridges in our model enzyme, Fusarium solani pisi cutinase, a lipolytic enzyme. With this purpose, we acquired transient absorption data of cutinase, with supplemental experimental data on tryptophan (Trp) and lysozyme as reference molecules. We here report formation kinetics and lifetimes of transient chemical species created upon UV excitation of aromatic residues in proteins. Two proteins, lysozyme and cutinase, as well as the free amino acid Trp, were studied under acidic, neutral, and alkaline conditions. The shortest-lived species is assigned to solvated electrons (lifetimes of a few microseconds to nanoseconds), whereas the longer-lived species are assigned to aromatic neutral and ionic radicals, Trp triplet states, and radic"@en . . . . "315196" . . . "Neves-Petersen, Maria Teresa" .