"V" . . "1" . "RIV/60076658:12640/08:00009970" . "391857" . "Pozad\u00ED: Poskytujeme nejnov\u011Bj\u0161\u00ED informace na\u0161\u00ED dvoufotonov\u00E9 laserov\u00E9 mikroskopie stla\u010Den\u00EDm nebo sn\u00ED\u017Een\u00EDm \u0161\u00ED\u0159ky pulsu pro disperzn\u00ED prekompenzaci, abychom umo\u017Enili puls\u016Fm proniknout hluboko dovnit\u0159 vzorku. V\u00FDsledky: Roz\u0161\u00ED\u0159en\u00ED p\u0159ich\u00E1z\u00ED, kdy\u017E pulsy projdou skrz optick\u00E9 prvky. Pozvedli jsme a modifikovali jsme kvalitu a ostrost zobrazen\u00ED zv\u00FD\u0161en\u00EDm rozli\u0161en\u00ED pou\u017Eit\u00EDm speci\u00E1ln\u00EDho polariz\u00E1toru Glan Laser polarizer GL10. Tento polariz\u00E1tor se skl\u00E1d\u00E1 ze dvou hranol\u016F odd\u011Blen\u00FDch vzdu\u0161n\u00FDm prostorem. Toto vzdu\u0161n\u00E9 odd\u011Blen\u00ED mezi dv\u011Bma hranoly se pou\u017E\u00EDv\u00E1 ke zpo\u017Ed\u011Bn\u00ED \u010Derven\u00E9 vlnov\u00E9 d\u00E9lky, kdy\u017E sv\u011Btlo opou\u0161t\u00ED prvn\u00ED hranol, proch\u00E1z\u00ED vzdu\u0161n\u00FDm prostorem a vch\u00E1z\u00ED do druh\u00E9ho hranolu. Pozorujeme zna\u010Dn\u00E9 zv\u011Bt\u0161en\u00ED s pou\u017Eit\u00EDm GL polariz\u00E1toru a m\u016F\u017Eeme vid\u011Bt detaily struktury v po\u010D\u00E1te\u010Dn\u00EDch etap\u00E1ch, kdy\u017E se sna\u017E\u00EDme dostat ohnisko skrz pr\u016Fb\u011Bh sn\u00EDm\u00E1n\u00ED v porovn\u00E1n\u00ED s m\u011B\u0159en\u00EDmi bez pou\u017Eit\u00ED GL polariz\u00E1toru. Proto s touto modifikac\u00ED jsme schopni redukovat expozi\u010Dn\u00ED \u010Das vzorku a pravd\u011Bpodobnost vysv\u00EDcen\u00ED a fototoxicity. Kdy\u017E je \u0161\u00ED\u0159ka pulsu re"@cs . "Reshak, Ali Hussain" . . "39" . "1"^^ . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Reduction of the pulse duration of the ultrafast laser pulses of the Two-Photon Laser Scanning Microscopy (2PLSM)" . . "Background: We provide an update of our two-photon laser scanning microscope by compressing or reducing the broadening of the pulse width of ultrafast laser pulses for dispersion precompensation, to enable the pulses to penetrate deeply inside the sample. Findings: The broadening comes as the pulses pass through the optical elements. We enhanced and modified the quality and the sharpness of images by enhancing the resolution using special polarizer namely Glan Laser polarizer GL10. This polarizer consist of two prisms separated by air space. This air separation between the two prisms uses to delay the red wavelenght when the light leaves the first prism to the air then to second prism. We note a considerable enhancing with using the GL polarizer, and we can see the details of structure in early stages when we trying to get focus through z-stacks of images in comparison to exactly the same measurements without using GL polarizer. Hence, with this modification we able to reduce the time of exposure the" . "Reshak, Ali Hussain" . "Reduction of the pulse duration of the ultrafast laser pulses of the Two-Photon Laser Scanning Microscopy (2PLSM)"@en . "RIV/60076658:12640/08:00009970!RIV09-MSM-12640___" . "Background: We provide an update of our two-photon laser scanning microscope by compressing or reducing the broadening of the pulse width of ultrafast laser pulses for dispersion precompensation, to enable the pulses to penetrate deeply inside the sample. Findings: The broadening comes as the pulses pass through the optical elements. We enhanced and modified the quality and the sharpness of images by enhancing the resolution using special polarizer namely Glan Laser polarizer GL10. This polarizer consist of two prisms separated by air space. This air separation between the two prisms uses to delay the red wavelenght when the light leaves the first prism to the air then to second prism. We note a considerable enhancing with using the GL polarizer, and we can see the details of structure in early stages when we trying to get focus through z-stacks of images in comparison to exactly the same measurements without using GL polarizer. Hence, with this modification we able to reduce the time of exposure the"@en . . . "[5A8D1E2A3EA4]" . "1756-0500" . "Reduction of the pulse duration of the ultrafast laser pulses of the Two-Photon Laser Scanning Microscopy (2PLSM)"@en . . . "12640" . "BMC Research Notes" . "7"^^ . "1"^^ . . "laser; Two-photon"@en . . . . "Redukce d\u00E9lky pulsu ultrarychl\u00FDch laserov\u00FDch puls\u016F dvoufotonov\u00E9 laserov\u00E9 mikroskopie (2PLSM)"@cs . "Redukce d\u00E9lky pulsu ultrarychl\u00FDch laserov\u00FDch puls\u016F dvoufotonov\u00E9 laserov\u00E9 mikroskopie (2PLSM)"@cs . . "Reduction of the pulse duration of the ultrafast laser pulses of the Two-Photon Laser Scanning Microscopy (2PLSM)" .