"Crystallographic study of Escherichia coli favoprotein WrbA, a new NAD(P)H-dependent guinine oxidoreductase"@en . "Flavoprotein WrbA z E.coli reprezentuje novou rodinu multimern\u00EDch protein\u016F podobn\u00FDch flavodoxin\u016Fm zahrnut\u00FDch v bun\u011B\u010Dn\u00E9 ochran\u011B proti oxidativn\u00EDmu stresu. V sou\u010Dasnosti odhalenou NAD(P)H-dependentn\u00ED chinon-oxidoredukt\u00E1zovou aktivitu podn\u011Bcuje ur\u010Den\u00ED krystalov\u00E9 struktury WrbA a n\u00E1sleduj\u00EDc\u00ED v\u00FDzkum strukturn\u00EDch rys\u016F charakterizuj\u00EDc\u00ED novou rodinu redoxn\u011B aktivn\u00EDch protein\u016F. Dv\u011B r\u016Fzn\u00E9 krystalov\u00E9 formy WrbA proteinu s nav\u00E1zan\u00FDm kofaktorem FMN byly z\u00EDsk\u00E1ny a n\u00E1sledn\u011B ur\u010Deny jejich krystalov\u00E9 struktury s rozli\u0161en\u00EDm 2.6 and 2.0 \u00C5. Z d\u016Fvodu ur\u010Dit vliv FMN nav\u00E1zan\u00E9ho do aktivn\u00EDho m\u00EDsta, apoprotein WrbA (bez nav\u00E1zan\u00E9ho FMN) byl krystalizov\u00E1n a difrak\u010Dn\u00ED data nam\u011B\u0159ena do rozli\u0161en\u00ED 1.85"@cs . "Wolfov\u00E1, Julie" . "Carey, J." . . "15" . . . . . "Kut\u00E1-Smatanov\u00E1, Ivana" . . . "6"^^ . . "Brynda, Ji\u0159\u00ED" . "RIV/60076658:12640/08:00009373!RIV09-MSM-12640___" . . "Mesters, J. R." . "3"^^ . "361647" . "1211-5894" . "The flavoprotein WrbA from Escherichia coli represents a new family of multimeric flavodoxin-like proteins implicated in cell protection against oxidative stress. The recently revealed NAD(P)H-dependent quinone oxidoreductase activity stimulated determination of crystal structures of E. coli WrbA and the folowing search for structural features characterizing the new family of redox-active proteins. Two different crystal forms were obtained for E. coli WrbA in complex with its flavin cofactor (FMN) and the crystal structures were determined to resolutions of 2.6 and 2.0\u00C5. In order to investigate influence of FMN binding on the protein structure, WrbA apoprotein (without FMN bound)was crystallized and the diffraction data were recorded to a resolution of 1.85" . . "12640" . "1" . "RIV/60076658:12640/08:00009373" . . "Crystallographic study of Escherichia coli favoprotein WrbA, a new NAD(P)H-dependent guinine oxidoreductase"@en . "Krystalografick\u00E1 studie flavoproteinu WrbA z E.coli, nov\u00E9 NAD(P)H-dependentn\u00ED chinon-oxidoreduktasy"@cs . . . "Materials Structure" . . "Crystallographic study of Escherichia coli favoprotein WrbA, a new NAD(P)H-dependent guinine oxidoreductase" . "The flavoprotein WrbA from Escherichia coli represents a new family of multimeric flavodoxin-like proteins implicated in cell protection against oxidative stress. The recently revealed NAD(P)H-dependent quinone oxidoreductase activity stimulated determination of crystal structures of E. coli WrbA and the folowing search for structural features characterizing the new family of redox-active proteins. Two different crystal forms were obtained for E. coli WrbA in complex with its flavin cofactor (FMN) and the crystal structures were determined to resolutions of 2.6 and 2.0\u00C5. In order to investigate influence of FMN binding on the protein structure, WrbA apoprotein (without FMN bound)was crystallized and the diffraction data were recorded to a resolution of 1.85"@en . . "Crystallographic study of Escherichia coli favoprotein WrbA, a new NAD(P)H-dependent guinine oxidoreductase" . . . "CZ - \u010Cesk\u00E1 republika" . . . "Grandori, R." . "P(LC06010), P(ME 640), S, Z(MSM6007665808)" . "[927C9068A132]" . "Biological crystallography; flavoprotein; oxidoreductase activity; single crystal growth; X-ray diffraction"@en . . . . . "Krystalografick\u00E1 studie flavoproteinu WrbA z E.coli, nov\u00E9 NAD(P)H-dependentn\u00ED chinon-oxidoreduktasy"@cs . "3"^^ .