. "RIV/60076658:12640/06:00006791" . . "Kombinatorick\u00E9 zobrazov\u00E1n\u00ED: Jak\u00FD protokol a kter\u00E9 chlorofylov\u00E9 fluorescen\u010Dn\u00ED obrazy pou\u017E\u00EDt pro vizualizaci infekce arabidopsis thaliana bakteri\u00ED pseudomonas syringae."@cs . . "biotic stress; chlorophyll fluorescence; classification; fluorescence induction; forced oscillations; image processing; image segmentation"@en . "V publikaci popisujeme problematiku detekce infekce arabidopsis thaliana bakteri\u00ED pseudomonas syringae pomoc\u00ED zobrazovac\u00ED chlorofylov\u00E9 fluorometrie. Porovn\u00E1v\u00E1me v\u00FDhody a mo\u017Enosti konven\u010Dn\u00EDho p\u0159\u00EDstupu a metody nazvan\u00E9 kombinatorick\u00E9 zobrazov\u00E1n\u00ED. Kombinatorick\u00E9 zobrazov\u00E1n\u00ED vyu\u017E\u00EDv\u00E1 v\u00FDhod metod selekce p\u0159\u00EDznak\u016F a klasifikace a umo\u017E\u0148uje detekovat infekci v\u00FDrazn\u011B d\u0159\u00EDve ne\u017E konven\u010Dn\u00EDm p\u0159\u00EDstupem. V\u00FDhody tohoto p\u0159\u00EDstupu jsou demonstrov\u00E1ny na schopnostech detekovat infekci na listech rostlin, detekovat jej\u00ED f\u00E1zi a schopnosti ohodnotit informa\u010Dn\u00ED p\u0159\u00EDnos jednotliv\u00FDch \u010D\u00E1st\u00ED m\u011B\u0159\u00EDc\u00EDho protokolu."@cs . . . "467817" . . . . . "3"^^ . . "Berger, Susanne" . "Photosynthesis Research" . "12640" . "Roitsch, Thomas" . "3" . . "S, Z(MSM6007665808)" . . "243-253" . "Case study of combinatorial imaging: What protocol and what chlorophyll fluorescence image to use when visualizing infection of Arabidopsis thaliana by Pseudomonas syringae?"@en . . "90" . "11"^^ . "Localized infection of a plant can be mapped by a sequence of images capturing chlorophyll fluorescence transients in actinic light. Choice of the actinic light protocol co-determines fluorescence contrast between infected leaf segment and surrounding healthy tissue. Frequently, biology cannot predict with which irradiance protocol, in which fluorescence image of the sequence, and in which segment of the image there will be the highest contrast between the healthy and infected tissue. Here, we introduce a new technique that can be applied to identify the combination of chlorophyll fluorescence images yielding the highest contrast. The sets of the most contrasting images vary throughout the progress of the infection. Such specific image sets, stress-revealing fluorescence signatures, can be found for the initial and late phases of the infection. Using these signatures, images can be divided into segments that show tissue in different infection phases. We demonstrate the capacity of the algorithm in an"@en . . . "Matou\u0161, Karel" . . "5"^^ . . "0166-8595" . . "Benediktyov\u00E1, Zuzana" . "Case study of combinatorial imaging: What protocol and what chlorophyll fluorescence image to use when visualizing infection of Arabidopsis thaliana by Pseudomonas syringae?" . "Localized infection of a plant can be mapped by a sequence of images capturing chlorophyll fluorescence transients in actinic light. Choice of the actinic light protocol co-determines fluorescence contrast between infected leaf segment and surrounding healthy tissue. Frequently, biology cannot predict with which irradiance protocol, in which fluorescence image of the sequence, and in which segment of the image there will be the highest contrast between the healthy and infected tissue. Here, we introduce a new technique that can be applied to identify the combination of chlorophyll fluorescence images yielding the highest contrast. The sets of the most contrasting images vary throughout the progress of the infection. Such specific image sets, stress-revealing fluorescence signatures, can be found for the initial and late phases of the infection. Using these signatures, images can be divided into segments that show tissue in different infection phases. We demonstrate the capacity of the algorithm in an" . "[8DA32D23A89C]" . "Case study of combinatorial imaging: What protocol and what chlorophyll fluorescence image to use when visualizing infection of Arabidopsis thaliana by Pseudomonas syringae?"@en . . "Case study of combinatorial imaging: What protocol and what chlorophyll fluorescence image to use when visualizing infection of Arabidopsis thaliana by Pseudomonas syringae?" . . "Kombinatorick\u00E9 zobrazov\u00E1n\u00ED: Jak\u00FD protokol a kter\u00E9 chlorofylov\u00E9 fluorescen\u010Dn\u00ED obrazy pou\u017E\u00EDt pro vizualizaci infekce arabidopsis thaliana bakteri\u00ED pseudomonas syringae."@cs . "Nedbal, Ladislav" . . "RIV/60076658:12640/06:00006791!RIV07-MSM-12640___" . . "NL - Nizozemsko" .