. "7"^^ . "RIV/60076658:12610/08:00008882!RIV09-MSM-12610___" . "0175-8659" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . "Z(MSM6007665809), Z(MSM6215712402)" . "Beklov\u00E1, M." . . . "Kizek, R." . "Adam, V." . "Stejskal, K." . . . . . "Svobodov\u00E1, Zde\u0148ka" . "4" . . . "3"^^ . "Fabrik, I." . "Glutathione; reduced glutathione; oxidized glutathione; cysteine; Acipenser baerii; Acipenser ruthenus; Perca fluviatilis; Sander lucioperca; spermatozoa"@en . "Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)" . "Glutation, redukovan\u00E1 forma, oxidova\u00E1 forma, cystein, Acipenser baerii, Acipenser ruthenus, Perca fluviatilis, Sander lucioperca, spermatozoa"@cs . "Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)"@en . . . "Glutation, redukovan\u00E1 forma, oxidova\u00E1 forma, cystein, Acipenser baerii, Acipenser ruthenus, Perca fluviatilis, Sander lucioperca, spermatozoa"@cs . . "[0363438F89CA]" . "Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)"@en . "24" . "Glutathione belongs to a vital intra- and extra-cellular protective antioxidant and is found almost exclusively in its reduced form. The ratio between its reduced and oxidized within cells is often used as a marker of cellular toxicity. The objectives of the study were to (i) determine both the reduced (GSH) and oxidized glutathione (GSSG) and cysteine (Cys) in the sperm of the Acipenser baerii and Acipenser ruthenus, as well as in Perca fluviatilis and Sander lucioperca, and (ii) to demonstrate the differences in concentration levels between representatives of acipenseriform and teleost species. High performance liquid chromatography with electrochemical detection was employed. The average content of the thiols determined in the sperm samples were as follows: Acipenser baerii (cysteine 55 ? 8 \u03BCg ml-1; GSH 126 ? 19 \u03BCg ml-1; GSSG 49 ? 7 \u03BCg ml-1), Acipenser ruthenus (cysteine 62 ? 9 \u03BCg ml-1; GSH 768 ? 115 \u03BCg ml-1; GSSG 180 ? 16 \u03BCg ml-1), Sander lucioperca (cysteine 251 ? 38" . "12610" . . . . "2"^^ . "Journal of Applied Ichthyology" . . . "RIV/60076658:12610/08:00008882" . "Glutation pat\u0159\u00ED mezi intra a extracelul\u00E1rn\u011B p\u016Fsob\u00EDc\u00ED protektivn\u00ED antioxidanty t\u00E9m\u011B\u0159 v\u00FDhradn\u011B pouze ve sv\u00E9 redukovan\u00E9 form\u011B. Pom\u011Br mezi redukovanou a oxidovanou formou uvnit\u0159 bu\u0148ky je \u010Dasto pou\u017E\u00EDv\u00E1n jako ukazatel bun\u011B\u010Dn\u00E9 toxicity. C\u00EDlem t\u00E9to studie bylo ur\u010Dit obsah obou (oxidovan\u00E9 i redukovan\u00E9) forem glutationu, a obsah cysteinu ve spermatu jesetera sibi\u0159sk\u00E9ho, jesetera mal\u00E9ho, okouna \u0159\u00ED\u010Dn\u00EDho a cand\u00E1ta obecn\u00E9ho, a demonstrovat rozd\u00EDly mezi z\u00E1stupci chrupav\u010Dit\u00FDch (jeseterovit\u00FDch) a kostnat\u00FDch ryb."@cs . . "000258458400026" . . "361140" . "Rodina, Marek" . . . "Glutathione belongs to a vital intra- and extra-cellular protective antioxidant and is found almost exclusively in its reduced form. The ratio between its reduced and oxidized within cells is often used as a marker of cellular toxicity. The objectives of the study were to (i) determine both the reduced (GSH) and oxidized glutathione (GSSG) and cysteine (Cys) in the sperm of the Acipenser baerii and Acipenser ruthenus, as well as in Perca fluviatilis and Sander lucioperca, and (ii) to demonstrate the differences in concentration levels between representatives of acipenseriform and teleost species. High performance liquid chromatography with electrochemical detection was employed. The average content of the thiols determined in the sperm samples were as follows: Acipenser baerii (cysteine 55 ? 8 \u03BCg ml-1; GSH 126 ? 19 \u03BCg ml-1; GSSG 49 ? 7 \u03BCg ml-1), Acipenser ruthenus (cysteine 62 ? 9 \u03BCg ml-1; GSH 768 ? 115 \u03BCg ml-1; GSSG 180 ? 16 \u03BCg ml-1), Sander lucioperca (cysteine 251 ? 38"@en . "Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)" . .