"[BE2FFD451843]" . "Karami, M." . "Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (Salmo trutta caspius)" . "Sperm density, mineral and organic composition of the seminal plasma and their physiological relationship were investigated in the Caspian brown trout (Salmo trutta caspius). To establish a rapid and accurate method for assessment of sperm density, three different techniques were used: sperm counting, spectrophotometry (at 480 nm) and determination of the spermatocrit. The seminal plasma contained 159.26 +/- 8.84mM sodium (Na), 33.72 +/- 2.01mM potassium (K), 133.04 +/- 5.96mM chlorine (Cl), 1.68 +/- 0.2mM calcium (Ca) and 0.988 +/- 0.13mM magnesium (Mg). The following organic components were found: total protein 0.75 +/- 0.14 mg100mL-1, cholesterol 2.86 +/- 0.58mg L-1 and glucose 3.81 +/- 1.04 mM L L-1. The mean sperm density was estimated to be 3.3 +/- 109 spermatozoam L-1. The spermatocrit (%) ranged from 25 to 52 in sperm samples. Highly significant linear relationships were found between sperm density and spermatocrit (R2=0.703, P<0.001) and sperm density and optical density (R2=0.909, P<0." . "451609" . "5"^^ . "12610" . "Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (Salmo trutta caspius)" . "Niksirat, H." . . . "38" . "Alavi, Sayyed Mohammad Hadi" . . "Sperm density, mineral and organic composition of the seminal plasma and their physiological relationship were investigated in the Caspian brown trout (Salmo trutta caspius). To establish a rapid and accurate method for assessment of sperm density, three different techniques were used: sperm counting, spectrophotometry (at 480 nm) and determination of the spermatocrit. The seminal plasma contained 159.26 +/- 8.84mM sodium (Na), 33.72 +/- 2.01mM potassium (K), 133.04 +/- 5.96mM chlorine (Cl), 1.68 +/- 0.2mM calcium (Ca) and 0.988 +/- 0.13mM magnesium (Mg). The following organic components were found: total protein 0.75 +/- 0.14 mg100mL-1, cholesterol 2.86 +/- 0.58mg L-1 and glucose 3.81 +/- 1.04 mM L L-1. The mean sperm density was estimated to be 3.3 +/- 109 spermatozoam L-1. The spermatocrit (%) ranged from 25 to 52 in sperm samples. Highly significant linear relationships were found between sperm density and spermatocrit (R2=0.703, P<0.001) and sperm density and optical density (R2=0.909, P<0."@en . . "Aquaculture Research" . "1175 - 1181" . . "1"^^ . "Koncentarce spermi\u00ED, slo\u017Een\u00ED semin\u00E1ln\u00ED plazmy a jejich vz\u00E1jemn\u00FD fyziologick\u00FD vztah u ohro\u017Een\u00E9ho kaspick\u00E9ho pstruha obecn\u00E9ho (Salmo trutta caspius)"@cs . "RIV/60076658:12610/07:00007886!RIV08-MSM-12610___" . . "Koncentarce spermi\u00ED, slo\u017Een\u00ED semin\u00E1ln\u00ED plasmy a jejich vz\u00E1jemn\u00FD fyziologick\u00FD vztah u ohro\u017Een\u00E9ho kaspick\u00E9ho pstruha obecn\u00E9ho (Salmo trutta caspius). Pro zaveden\u00ED rychl\u00E9ho a p\u0159esn\u00E9ho stanoven\u00ED koncentrace spermi\u00ED byly vyzkou\u0161eny t\u0159i odli\u0161n\u00E9 techniky: po\u010D\u00EDt\u00E1n\u00ED spermi\u00ED, spektrofotometrie (vlnov\u00E1 d\u00E9lka 480 nm) a stanoven\u00ED spermatokrytu. Semin\u00E1ln\u00ED plasma obsahovala sod\u00EDk (Na) v koncentraci 159,26 +/- 8,84 mM/L, drasl\u00EDk (K) v koncentraci 33,72 +/- 2.01 mM/L, chl\u00F3r (Cl) v koncentraci 133,04 +/- 5,96 mM/L, v\u00E1pn\u00EDk (Ca) v koncentraci 1,68 +/- 0,2 mM/L a ho\u0159\u010D\u00EDk (Mg) v koncentraci 0,988 +/- 0,13 mM/L. D\u00E1le byly v semin\u00E1ln\u00ED plasm\u011B obsa\u017Eeny n\u00E1sleduj\u00EDc\u00ED oragnick\u00E9 l\u00E1tky: celkov\u00FD protein 7,5 +/- 1,4 mg/L, cholesterol 2,86 +/- 0,58mg/L1 a gluk\u00F3za 3,81 +/- 1,04 mM/L. Pr\u016Fm\u011Brn\u00E1 koncentrace spermi\u00ED byla 3,3.109 spermi\u00ED / L. Spermatokryt se pohyboval od 25-52 %. Vysok\u00E1 line\u00E1rn\u00ED z\u00E1vislost byla zji\u0161t\u011Bna mezi koncentrac\u00ED spermi\u00ED zji\u0161t\u011Bnou po\u010D\u00EDt\u00E1n\u00EDm a spermatokrytem (R2=0,703, P<0,001) a mezi koncentrac\u00ED spermi\u00ED zji\u0161t\u011Bnou po"@cs . "Sperm density; seminal plasma; Caspian brown trout; mineral and organic components"@en . . "7"^^ . . . "Koncentarce spermi\u00ED, slo\u017Een\u00ED semin\u00E1ln\u00ED plazmy a jejich vz\u00E1jemn\u00FD fyziologick\u00FD vztah u ohro\u017Een\u00E9ho kaspick\u00E9ho pstruha obecn\u00E9ho (Salmo trutta caspius)"@cs . . "11" . . "Mojazi Amiri, B." . "RIV/60076658:12610/07:00007886" . . "Z(MSM6007665809)" . "Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (Salmo trutta caspius)"@en . . "Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (Salmo trutta caspius)"@en . "1355-557X" . . "Hatef, A." . "Alavi, Sayyed Mohammad Hadi" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . .