"RIV/60076658:12610/07:00007840" . . . "0044-8486" . . . "12610" . "Gela, David" . "272" . . "NL - Nizozemsko" . . "Imobilizing and cryopreservation of tench (Tinca tinca) sperm" . . . "Imobilizace a kryokonzervace spermatu l\u00EDna (Tinca tinca L.)"@cs . . . "Aquaculture" . "[FAB7AF98188E]" . . "In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian tench during three experiments. Sperm was collected directly into immobilizing solution of Kurokura and equilibrated at 4\u00B0C for 2,5 h. Collected sperm was transferred to 2 ml cryotubes or straws (0.5, 1 and 5 ml) and and cryoprotectants as DMSO or mixture of DMSO with propandiol were added. Than the cryotubes /straws were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled ether using a long cooling programme K4 (from +4\u00B0C to -9\u00B0C at a rate of 4\u00B0C . min-1, then from -9\u00B0C to -80\u00B0C at a rate of 11\u00B0C . min-1, held for 6 min at -80\u00B0C), or a rapid cooling programme L1 (from +4\u00B0C to -80\u00B0C at a rate of 20\u00B0C . min-1, held for 6 min at -80\u00B0C) and finally transferred into liquid N2. The spermatozoa were thwed in water bath 40\u00B0C according to the frozen volume and checked for fertilization and hatching rates. Sperm motility was evaluated for the percentage and velocity of motile spermatozoa from vi"@en . . "Imobilizing and cryopreservation of tench (Tinca tinca) sperm" . "P(GA524/06/0817)" . . "305" . "Imobilizing and cryopreservation of tench (Tinca tinca) sperm"@en . "1"^^ . "RIV/60076658:12610/07:00007840!RIV08-GA0-12610___" . "425533" . "In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian tench during three experiments. Sperm was collected directly into immobilizing solution of Kurokura and equilibrated at 4\u00B0C for 2,5 h. Collected sperm was transferred to 2 ml cryotubes or straws (0.5, 1 and 5 ml) and and cryoprotectants as DMSO or mixture of DMSO with propandiol were added. Than the cryotubes /straws were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled ether using a long cooling programme K4 (from +4\u00B0C to -9\u00B0C at a rate of 4\u00B0C . min-1, then from -9\u00B0C to -80\u00B0C at a rate of 11\u00B0C . min-1, held for 6 min at -80\u00B0C), or a rapid cooling programme L1 (from +4\u00B0C to -80\u00B0C at a rate of 20\u00B0C . min-1, held for 6 min at -80\u00B0C) and finally transferred into liquid N2. The spermatozoa were thwed in water bath 40\u00B0C according to the frozen volume and checked for fertilization and hatching rates. Sperm motility was evaluated for the percentage and velocity of motile spermatozoa from vi" . . . . "Cryopreservation; hatching rate; Tench Tinca tinca; sperm motility"@en . . "4"^^ . "Imobilizing and cryopreservation of tench (Tinca tinca) sperm"@en . "Rodina, Marek" . "Kocour, Martin" . . "V t\u00E1to pr\u00E1ci byla zpracov\u00E1v\u00E1na metoda kryokonzervace spermatu l\u00EDna obecn\u00E9ho pro pot\u0159eby ochrany genofondu. Sperma bylo odeb\u00EDr\u00E1no p\u0159\u00EDmo do Kurokurova imobiliza\u010Dn\u00EDho roztoku a uchov\u00E1no p\u0159i +4\u00B0C 2,5 hodiny. Ke spermatu byl p\u0159id\u00E1n kryoprotektant, sperma bylo napln\u011Bno do 2 ml kryozkumavek nebo pejet (o objemu 0,5, 1 a 5 ml) a pot\u00E9 byly kryozkumavky a pejety p\u0159eneseny do p\u0159edprogramovan\u00E9ho zmrazovac\u00EDho automatu PLANER Kryo 10 serie III a mra\u017Eeny del\u0161\u00EDm zmrazovac\u00EDm programem K4 (z teploty +4\u00B0C na -9\u00B0C rychlost\u00ED 4\u00B0C za minutu, z teploty -9\u00B0C na teplotu -80\u00B0C rychlost\u00ED 11\u00B0C za minutu, v\u00FDdr\u017E na -80\u00B0C 6 min) nebo rychlej\u0161\u00EDm programem L1 ( z +4\u00B0C na -80\u00B0C rychlost\u00ED 20\u00B0C za minutu, v\u00FDdr\u017E na -80\u00B0C 6 min). Potom byly d\u00E1vky p\u0159eneseny do tekut\u00E9ho dus\u00EDku. Sperma bylo rozmrazov\u00E1no na vodn\u00ED l\u00E1zni teploty 40\u00B0C po dobu podle objemu d\u00E1vky a pou\u017Eito pro test fertility na jikr\u00E1ch. Motilita a rychlost pohybu spermi\u00ED byla zji\u0161\u0165ov\u00E1na z videoz\u00E1znamu mikroskopick\u00E9ho obrazu pohybu spermi\u00ED. ANOVA ve v\u0161ech p\u0159\u00EDpadech vliv zmra\u017Eov\u00E1n\u00ED."@cs . "4"^^ . "1" . . "Imobilizace a kryokonzervace spermatu l\u00EDna (Tinca tinca L.)"@cs . "Linhart, Otomar" .