"12310" . . . "1" . . . . . "Nirmala, X." . "0014-2956" . "Insect silk contains both a Kunitz-type and a unique Kazal-type proteinase inhibitor"@en . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "RIV/60076658:12310/01:00003155!RIV/2002/GA0/123102/N" . . "2064-2073" . . "Insect silk contains both a Kunitz-type and a unique Kazal-type proteinase inhibitor" . . . . . . "683068" . . "In addition to the fibrous (fibroins) and sticky (sericins) components, the silk contains several small peptides of unknown function. We demonstrate that two of the small peptides present in the silk of the waxmoth, Galleria mellonella, inhibit proteolytic enzymes, and are thus called silk proteinase inhibitors 1 and 2 (SPI 1, SPI 2). They are produced in the middle section of the silk-secreting glands prior to cocoon spinning and their production is controlled at the transcription level. The full length cDNA of pre-SPI 1 contains 443 nt and encodes a peptide of 76 amino acid residues, of which 20 make up a signal sequence. The mature SPI 1 (6056.7 Da, 56 residues) is a typical thermostable Kunitz-type proteinase inhibitor with Arg in P1 position. The cDNA of pre-SPI 2 consists of 260 nt and yields a putative secretory peptide of 58 amino acid residues. The functional SPI 2 (3993 Da, 36 residues), generated after the signal sequence removal, is a single-domain Kazal-type proteinase inhibitor with uniq"@en . . . "268" . "[5A94359DE953]" . . "Sehnal, Franti\u0161ek" . "In addition to the fibrous (fibroins) and sticky (sericins) components, the silk contains several small peptides of unknown function. We demonstrate that two of the small peptides present in the silk of the waxmoth, Galleria mellonella, inhibit proteolytic enzymes, and are thus called silk proteinase inhibitors 1 and 2 (SPI 1, SPI 2). They are produced in the middle section of the silk-secreting glands prior to cocoon spinning and their production is controlled at the transcription level. The full length cDNA of pre-SPI 1 contains 443 nt and encodes a peptide of 76 amino acid residues, of which 20 make up a signal sequence. The mature SPI 1 (6056.7 Da, 56 residues) is a typical thermostable Kunitz-type proteinase inhibitor with Arg in P1 position. The cDNA of pre-SPI 2 consists of 260 nt and yields a putative secretory peptide of 58 amino acid residues. The functional SPI 2 (3993 Da, 36 residues), generated after the signal sequence removal, is a single-domain Kazal-type proteinase inhibitor with uniq" . "3"^^ . . "RIV/60076658:12310/01:00003155" . "Insect silk contains both a Kunitz-type and a unique Kazal-type proteinase inhibitor"@en . . "Kodr\u00EDk, Dalibor" . "10"^^ . "Insect silk contains both a Kunitz-type and a unique Kazal-type proteinase inhibitor" . "0"^^ . "4"^^ . "P(GA204/00/0019), P(ME 204)" . "0"^^ . "antimicrobial defense; Galleria mellonella; Kazal domain; proteinase inhibitors; silk"@en . "\u017Durovec, Michal" . "European Journal of Biochemistry" .