"RIV/60076658:12220/04:00005201!RIV/2005/GA0/122205/N" . "The effects of environmental conditions (year, growing site) on protein compounds in buckwheat (Fagopyrum esculentum Moench) were studied. Small-plot experiments with four buckwheat cultivars (tetraploid Emka and diploid Kora, Pyra, and Krupinka) were established in two growing sites in 1999 and 2000. Crude and pure protein content, proportion of protein solubility fractions, SDS-PAGE of protein fractions, and immunoassay of gluten-like proteins were determined in buckwheat flour. The growing site had the most significant effect on variability of crude protein content. On the contrary, pure protein content was most of all affected by year. Apparently a higher occurrence of later developed buckwheat seeds in 1999 harvest (as a consequence of weather abnormalities) could generally cause lower content of pure protein in this year (7.32 %) than in 2000 (9.10 %). Alcohol-soluble protein fraction (prolamins like proteins, PLP), considered as inconvenient for gluten-free diet, was generally found at low leve" . "HU - Ma\u010Farsko" . . "[63DB7F5D823F]" . "Kalinov\u00E1, Jana" . . . "Effects of environmental factors on protein content and composition in buckwheat flour" . . . . . "541-548" . "4"^^ . . "Effects of environmental factors on protein content and composition in buckwheat flour" . . . . "Cereal Research Communications" . "4"^^ . "V p\u0159\u00EDsp\u011Bvku byly studov\u00E1ny vlivy podm\u00EDnek prost\u0159ed\u00ED (ro\u010Dn\u00EDk, stanovi\u0161t\u011B) na b\u00EDlkovinn\u00E9 slo\u017Eky pohanky (Fagopyrum esculentum Moench). V letech 1999 a 2000 byly zalo\u017Eeny maloparcelkov\u00E9 pokusy se \u010Dty\u0159mi odr\u016Fdami pohanky (tetraploidn\u00ED Emka a diploidn\u00ED Kora, Pyra a Krupinka na dvou p\u011Bstitelsk\u00FDch stanovi\u0161t\u00EDch. Po sklizni byly v pohankov\u00E9 mouce analyzov\u00E1ny: obsah hrub\u00FDch a \u010Dist\u00FDch b\u00EDlkovin, proporce b\u00EDlkovinn\u00FDch frakc\u00ED dle rozpustnosti, b\u00EDlkovinn\u00E9 frakce pomoc\u00ED SDS-PAGE a byly provedeny imunochemick\u00E9 testy na obsah gluten-like protein\u016F. P\u011Bstitelsk\u00E9 stanovi\u0161t\u011B m\u011Blo nejv\u00FDznamn\u011Bj\u0161\u00ED vliv na variabilitu obsahu hrub\u00FDch b\u00EDlkovin. Naopak obsah \u010Dist\u00E9ho proteinu byl v\u011Bt\u0161inou ovlivn\u011Bn ro\u010Dn\u00EDkem. Patrn\u011B vy\u0161\u0161\u00ED v\u00FDskyt pozd\u011Bji vyvinut\u00FDch semen p\u0159i sklizni v roce 1999 (jako n\u00E1sledek abnormalit po\u010Das\u00ED) obecn\u011B mohl tvo\u0159it ni\u017E\u0161\u00ED obsah \u010Dist\u00E9ho proteinu v tomto roce (7.32 %) ne\u017E v roce 2000 (9.10 %). Frakce b\u00EDlkovin rozpustn\u00FDch v alkoholu (prolaminu podobn\u00E9 proteiny, PLP), kter\u00E1 je pova\u017Eov\u00E1na za nevhodnou pro gluten-free diety, b"@cs . . "4" . . "562163" . "0133-3720" . "RIV/60076658:12220/04:00005201" . . "P(GA303/01/1380), Z(MSM 122200002)" . "Vlivy p\u011Bstitelsk\u00E9ho prost\u0159ed\u00ED na obsah a slo\u017Een\u00ED b\u00EDlkovin v mouce pohanky"@cs . . . . "Effects of environmental factors on protein content and composition in buckwheat flour"@en . . "32" . "\u010Curn, Vladislav" . "12220" . "B\u00E1rta, Jan" . "Moudr\u00FD, Jan" . . . "Fagopyrum esculentum Moench;crude protein;pure protein;protein solubility fraction"@en . "Vlivy p\u011Bstitelsk\u00E9ho prost\u0159ed\u00ED na obsah a slo\u017Een\u00ED b\u00EDlkovin v mouce pohanky"@cs . "Effects of environmental factors on protein content and composition in buckwheat flour"@en . "The effects of environmental conditions (year, growing site) on protein compounds in buckwheat (Fagopyrum esculentum Moench) were studied. Small-plot experiments with four buckwheat cultivars (tetraploid Emka and diploid Kora, Pyra, and Krupinka) were established in two growing sites in 1999 and 2000. Crude and pure protein content, proportion of protein solubility fractions, SDS-PAGE of protein fractions, and immunoassay of gluten-like proteins were determined in buckwheat flour. The growing site had the most significant effect on variability of crude protein content. On the contrary, pure protein content was most of all affected by year. Apparently a higher occurrence of later developed buckwheat seeds in 1999 harvest (as a consequence of weather abnormalities) could generally cause lower content of pure protein in this year (7.32 %) than in 2000 (9.10 %). Alcohol-soluble protein fraction (prolamins like proteins, PLP), considered as inconvenient for gluten-free diet, was generally found at low leve"@en . "8"^^ . .