"Sonication assisted Agrobacterium-mediated transformation enhances the transformation efficiency in flax (Linum usitatissimum)" . "2007-01-01+01:00"^^ . "RIV/60076658:12110/07:00007491" . . "sonication; SAAT; genetic transformation; flax"@en . . . "[3977F837FF51]" . "V\u00E1vrov\u00E1, Zuzana" . "Sonication assisted Agrobacterium-mediated transformation enhances the transformation efficiency in flax (Linum usitatissimum)"@en . "12110" . . . "1"^^ . "\u00DAstav genetiky a biotechnol\u00F3gi\u00ED rastl\u00EDn SAV" . . "Sonication assisted Agrobacterium-mediated transformation enhances the transformation efficiency in flax (Linum usitatissimum)"@en . . "978-80-89088-51-5" . . . . "Skalick\u00FD, Tom\u00E1\u0161" . "Beranov\u00E1, Martina" . "451070" . . . "Sonication assisted Agrobacterium-mediated transformation enhances the transformation efficiency in flax (Linum usitatissimum)" . . "Star\u00E1 Lesn\u00E1, Slovakia" . . . "126" . . "Plant Biotechnology: Impact on High Quality Plant Production. Book of Abstracts" . "1"^^ . "U\u017Eit\u00ED sonikace p\u0159i transformac\u00EDch zprost\u0159edkovan\u00FDch agrobakteriem zvy\u0161uje \u00FA\u010Dinnost transforma\u010Dn\u00EDho procesu u lnu (Linum usitatissimum)"@cs . "U\u017Eit\u00ED sonikace p\u0159i transformac\u00EDch zprost\u0159edkovan\u00FDch agrobakteriem zvy\u0161uje \u00FA\u010Dinnost transforma\u010Dn\u00EDho procesu u lnu (Linum usitatissimum)"@cs . "P(1M06030), P(1P05ME800)" . "RIV/60076658:12110/07:00007491!RIV08-MSM-12110___" . "Vyu\u017Eit\u00ED bakteri\u00ED Agrobacterium tumefaciens v transforma\u010Dn\u00EDch syst\u00E9mech zalo\u017Een\u00FDch na jejich Ti plazmidech se stalo hlavn\u00EDm n\u00E1strojem pro inzerce ciz\u00EDch gen\u016F do rostlinn\u00FDch bun\u011Bk, kter\u00E9 posl\u00E9ze exprimuj\u00ED po\u017Eadovan\u00E9 genov\u00E9 produkty. Av\u0161ak u n\u011Bkter\u00FDch druh\u016F, ke kter\u00FDm n\u00E1le\u017E\u00ED tak\u00E9 len (Linum usitatissimum), dos\u00E1hly b\u011B\u017En\u00E9 transforma\u010Dn\u00ED postupy pom\u011Brn\u011B omezen\u00FDch v\u00FDsledk\u016F. V ned\u00E1vn\u00E9 dob\u011B bylo uk\u00E1z\u00E1no, \u017Ee sonikac\u00ED zprost\u0159edkovan\u00E1 agrobakteri\u00E1ln\u00ED transformace (SAAT) v\u00FDrazn\u011B zvy\u0161uje \u00FArove\u0148 transientn\u00ED exprese report\u00E9rov\u00E9ho genu GUS a frekvence transformac\u00ED u n\u011Bkter\u00FDch rostlin\u00FDch druh\u016F. Vzhledem k odli\u0161nosti odezvy r\u016Fzn\u00FDch pletiv na SAAT, je t\u0159eba optimalizovat transforma\u010Dn\u00ED protokoly pro jednotliv\u00E1 pletiva. V t\u00E9to pr\u00E1ci bylo vyu\u017Eito SAAT ke zv\u00FD\u0161en\u00ED \u00FA\u010Dinnosti transformace hypokotylov\u00FDch explant\u00E1t\u016F lnu."@cs . . "3"^^ . "The use of Agrobacterium tumefaciens in transformation system based on its Ti plazmid has become the major tool for insertion of foreign genes into plant cells which then express desirable gene products. However, in certain species, to which belongs also flax (Linum usitatissimum), the transformation methods has reached rather slow progress and mostly has lead to unsatisfactory results. Recently, it has been shown that sonication assisted Agrobacterium-mediated transformation (SAAT) greatly enhanced the levels of transient expression of a GUS reporter gene and transformation frequencies in some species. As tissues differ in their response to SAAT, each plant tissue needs the optimized protocol. Here, the SAAT was used to increase transformation efficiencies for flax hypocotyls."@en . "The use of Agrobacterium tumefaciens in transformation system based on its Ti plazmid has become the major tool for insertion of foreign genes into plant cells which then express desirable gene products. However, in certain species, to which belongs also flax (Linum usitatissimum), the transformation methods has reached rather slow progress and mostly has lead to unsatisfactory results. Recently, it has been shown that sonication assisted Agrobacterium-mediated transformation (SAAT) greatly enhanced the levels of transient expression of a GUS reporter gene and transformation frequencies in some species. As tissues differ in their response to SAAT, each plant tissue needs the optimized protocol. Here, the SAAT was used to increase transformation efficiencies for flax hypocotyls." . .